An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

Sabrina Shameen Alam; Laila Khaleda; Mohammad Al-Forkan

An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

Sabrina Shameen Alam * §

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Laila Khaleda

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Mohammad Al-Forkan

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University of Chittagong

An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7

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Abstract

An efficient in vitro regeneration system for Tori (Brassica campestris) -7 was developed from calli of hypocotyls and cotyledonary leaves. The optimum medium for callus induction was found with 0.5 mgl -1 2, 4-dichloro-phenoxyacetic acid (2,4-D). The best shooting medium contained 3.0 mgl-1 6, benzyl amino purine (BAP), 0.1 mgl -1 naphtalene acetic acid (NAA), and 5.0 mgl -1 AgNO 3 . Maximum number of shoots were produced when 0.5 mgl -1 kinetin was used, whereas the combined effect of 2.0 mgl -1 BAP, 0.1 mgl -1 NAA, and 5.0 mgl -1 AgNO 3 regenerated calli the most. The effect of AgNO 3 was found for callogenesis at 0.5 mgl -1 and for regeneration at 5.0 mgl -1 . In vitro regenerated shoots of Tori-7 developed roots on medium with 1.0 mgl -1 indole 3-butyric acid (IBA). The developed efficient in vitro regeneration protocol can be used as a baseline for Agrobacterium-mediated genetic transformation of the studied plants.

References

12 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Sabrina Shameen Alam, Laila Khaleda, Mohammad Al-Forkan. 2013. "An Efficient in Vitro Regeneration System for Tori (Brassica Campestris)-7". Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 13 (GJSFR Volume 13 Issue G2).

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GJSFR Volume 13 Issue G2

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

agrobacterium and tori-7 in vitro regeneration

Classification

GJSFR-G Classification FOR Code: 270805

Submission ReceivedSeptember 7, 2013
Peer Review Double Blind
Handling Editor
Accepted September 27, 2013
Published October 7, 2013

Version of record

v1.2

Issue date

October 30, 2013

Language

English

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Article Matrices

Total Score: 147

Country: Bangladesh

Subject: Global Journal of Science Frontier Research

Authors: Sabrina Shameen Alam, Laila Khaleda, Mohammad Al-Forkan (PhD/Dr. count: 0)

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Publish Date: 2013 10, Mon

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