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ReserarchID
PDDTMR2U42
Crossref Journal DOI 10.17406/gjmra
Print ISSN 0975-5888
e-ISSN 2249-4618
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Biofilm represents the most prevalent type of virulent factor of most of the pathogenic microorganism and involved in crucial development of clinical infection and exhibit resistance to antimicrobial agents. Now the biofilm is considered as major target for the pharmacological development of drugs. A biofilm serves to promote bacteria persistence by resisting antibiotic treatment and host immune responses. Antibiotics are rendered ineffective when biofilms form due to their relative impermeability, the variable physiological status of microorganisms, subpopulations of persistent strains, and variations of phenotypes present. Metal nanotechnology chemistry has the potential to prevent the formation of these life-threatening biofilms on life supporting devices. In the present study, anti biofilm effect of silver nanoparticles coated catheter against clinical isolate of Staphylococcus Aureus was studied. Silver nanoparticles synthesized by leaf extract broth of Azadirhacta indica were coated on the catheter chara-cterized by scanning electron microscopy which reveals complete dispersion of the nanoparticles on the fibre surface of the catheter and the size, shape of the particles shows uniform spherical particles with the size of 50-60 nm. Distinct effect of biofilm inhibition was recorded in the nanoparticles coated catheter and maximum inhibition was observed during 72 hour of incubation. Biochemical composition of biofilm matrix mainly total carbohydrates and total protein was highly reduced. The present study would suggests the development of anti microbial coated medical devices against pathogenic microorganism.
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