Characterization and Antibiotics SensitivityPatternof Enterobacteriaceae in Obafemi Awolowo University Sewage Oxidation Pond

1
Sokan-Adeaga Micheal Ayodeji
Sokan-Adeaga Micheal Ayodeji
2
Sokan-Adeaga Adewale Allen
Sokan-Adeaga Adewale Allen
3
Sokan-Adeaga Eniola Deborah
Sokan-Adeaga Eniola Deborah
1 Obafemi Awolowo University

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Some members of the family Enterobacteriaceae were isolated from Obafemi Awolowo University sewage oxidation pond (target site) and characterize by various biochemical test (citrate test, Gram stain, catalase test, idole production, fermentation of sugar, starch hydrolysis, Nitrate reduction, oxidative-fermentative test, gelatin hydrolysis and Methyl Red Voges-Proskauer test); the antibiotic sensitivity pattern of the isolate were also carried out. All was done under an aseptic condition. The total bacterial count (TBC) obtained from the target site was higher than those obtained from the relative site (1: before the sewage enter) and (2: after leaving) the oxidation pond. The biochemical tests identified the following: Citrobacter diversus, Salmonella arizonae, Typical Salmonella, Escherichia coli and Providencia alcalifaciens, all belonging to the family Enterobacteriaceae. Investigation of the antibiotic sensitivity test revealed that Cefuroxime and Ampicillin were not effective against all the bacteria isolates.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Sokan-Adeaga Micheal Ayodeji. 2018. \u201cCharacterization and Antibiotics SensitivityPatternof Enterobacteriaceae in Obafemi Awolowo University Sewage Oxidation Pond\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 18 (GJMR Volume 18 Issue C1): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-C Classification: NLMC Code: QW 4
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July 30, 2018

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Some members of the family Enterobacteriaceae were isolated from Obafemi Awolowo University sewage oxidation pond (target site) and characterize by various biochemical test (citrate test, Gram stain, catalase test, idole production, fermentation of sugar, starch hydrolysis, Nitrate reduction, oxidative-fermentative test, gelatin hydrolysis and Methyl Red Voges-Proskauer test); the antibiotic sensitivity pattern of the isolate were also carried out. All was done under an aseptic condition. The total bacterial count (TBC) obtained from the target site was higher than those obtained from the relative site (1: before the sewage enter) and (2: after leaving) the oxidation pond. The biochemical tests identified the following: Citrobacter diversus, Salmonella arizonae, Typical Salmonella, Escherichia coli and Providencia alcalifaciens, all belonging to the family Enterobacteriaceae. Investigation of the antibiotic sensitivity test revealed that Cefuroxime and Ampicillin were not effective against all the bacteria isolates.

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Characterization and Antibiotics SensitivityPatternof Enterobacteriaceae in Obafemi Awolowo University Sewage Oxidation Pond

Sokan-Adeaga Micheal Ayodeji
Sokan-Adeaga Micheal Ayodeji Obafemi Awolowo University
Sokan-Adeaga Adewale Allen
Sokan-Adeaga Adewale Allen
Sokan-Adeaga Eniola Deborah
Sokan-Adeaga Eniola Deborah

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