Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples

1
Hussein Mukasa Kafeero
Hussein Mukasa Kafeero
2
Frank Nobert Mwiine
Frank Nobert Mwiine
3
David Atuhaire Kalenzi
David Atuhaire Kalenzi
4
Sylvester Ochwo
Sylvester Ochwo
5
Ann Nanteza
Ann Nanteza
1 Makerere University,

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Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples Banner
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Foot-and-mouth disease (FMD) is a viral disease of Ungulates; both Artiodactyla and Perissodactyla. The mortality rates are low in adult animals but it affects milk yield and international trade. In endemic countries, diagnosis can be based on clinical signs. But these are shared by other vesicular diseases, so a laboratory is needed to confirm the disease. In Uganda the commonly used assays for the laboratory diagnosis of FMD are NSP ELISA and RT-PCR. Serology using ELISA techniques may fail to distinguish between vaccinated and new infection so compromising its sensitivity. The gel passed PCR is involves a lot of advance sample treatment increasing errors due to carry over which also compromises its sensitivity. This work reports comparative the detection of foot-and-mouth virus by NSP ELISA and RT-PCR with real time PCR which was taken as the gold standard. The assays were compared in terms of sensitivity, specificity and disease prevalence and likelihood ratios. A total of 176 cattle were used from which samples that included epithelial tissues (17.05%) and oral swabs (84.09%) were collected from outbreak cases in Eastern Districts of Mbale and Budaka.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Hussein Mukasa Kafeero. 2016. \u201cComparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples\u201d. Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 16 (GJMR Volume 16 Issue G2): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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August 31, 2016

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Foot-and-mouth disease (FMD) is a viral disease of Ungulates; both Artiodactyla and Perissodactyla. The mortality rates are low in adult animals but it affects milk yield and international trade. In endemic countries, diagnosis can be based on clinical signs. But these are shared by other vesicular diseases, so a laboratory is needed to confirm the disease. In Uganda the commonly used assays for the laboratory diagnosis of FMD are NSP ELISA and RT-PCR. Serology using ELISA techniques may fail to distinguish between vaccinated and new infection so compromising its sensitivity. The gel passed PCR is involves a lot of advance sample treatment increasing errors due to carry over which also compromises its sensitivity. This work reports comparative the detection of foot-and-mouth virus by NSP ELISA and RT-PCR with real time PCR which was taken as the gold standard. The assays were compared in terms of sensitivity, specificity and disease prevalence and likelihood ratios. A total of 176 cattle were used from which samples that included epithelial tissues (17.05%) and oral swabs (84.09%) were collected from outbreak cases in Eastern Districts of Mbale and Budaka.

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Comparative Detection of Foot-and-Mouth Disease Virus by the two Commonly used Assays of NSP ELISA and RT-PCR In Uganda with Quantitative Real Time RT-PCR on Field Samples

Hussein Mukasa Kafeero
Hussein Mukasa Kafeero Makerere University,
Frank Nobert Mwiine
Frank Nobert Mwiine
David Atuhaire Kalenzi
David Atuhaire Kalenzi
Sylvester Ochwo
Sylvester Ochwo
Ann Nanteza
Ann Nanteza

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