Comparison of Two Methods in the Detection of Cryptosporidium in Pigs in Ogun State, Nigeria

Akinkuotu Olufemi Ambrose; Jacobs Eniope Bamidele; Okwelum Ngozi

Comparison of Two Methods in the Detection of Cryptosporidium in Pigs in Ogun State, Nigeria

Akinkuotu Olufemi Ambrose * §

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Jacobs Eniope Bamidele

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Okwelum Ngozi

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§ Federal University of Agriculture, Abeokuta, Ogun St...

Comparison of Two Methods in the Detection of Cryptosporidium in Pigs in Ogun State, Nigeria

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Abstract

Two diagnostic methods, a modified Kinyoun’s acid-fast staining technique and an enzyme-linked immunosorbent assay (ELISA), for the detection of Cryptosporidium spp. in porcine faeces were compared regarding their sensitivities. Of the 209 faecal samples examined, Cryptosporidium spp. was detected significantly higher (p

References

24 Cites in Article

Reference Format

A Ayinmode,B Fagbemi (2010). Prevalence of Cryptosporidium infection in cattle from southern Nigeria.
Derrick Baxby,N Blundell (1983). SENSITIVE, RAPID, SIMPLE METHODS FOR DETECTING CRYPTOSPORIDIUM IN FAECES.
M Bergeland (1977). Necrotic Enteritis in Nursing Piglets.
Rachel Chalmers,Brian Campbell,Nigel Crouch,André Charlett,Angharad Davies (2011). Comparison of diagnostic sensitivity and specificity of seven Cryptosporidium assays used in the UK.
F Chen,K Huang (2007). Prevalence and phylogenetic analysis of Cryptosporidium in pigs in eastern China.
Z Chen,R Mi,H Yu,Y Shi,Y Huang,Y Chen,P Zhou,Y Cai,P Lin (2011). Prevalence of Cryptosporidium spp. in pigs in Shanghai, China.
A El-Moamly,M El-Sweify (2011). ImmunoCard STAT! cartridge antigen detection assay compared to microplate enzyme immunoassay and modified Kinyoun's acid-fast staining technique for detection of Cryptosporidium in fecal specimens.
A El-Shazly,A Gabr,M Mahmoud,S Aziz,W Saleh (2002). The use of Ziehl-Neelsen stain, enzyme-linked immunosorbent assay and nested Polymerase Chain Reaction in diagnosis of cryptosporidiosis in immunocompetent, compromised patients.
A Godekmerdan,A Kalkan,A Erensoy,S Kilic (1999). Prevalence of Cryptosporidium spp. in children with diarrhoea.
In,H Yilmaz,T Zeynep,C Mautalip (2008). Enzyme-linked immunosorbent assay.
Y Hamedi,O Safa,M Haidari (2005). Cryptosporidium infection in diarrhoeic children in southeastern Iran.
U Ibrahim,A Mbaya,H Mahmud,A Mohamed (2007). Prevalence of cryptosporidiosis among captive wild animals and birds in the arid region of North-eastern Nigeria.
Ignatius Eisenblätter,M Regnath,T Mansmann,U Futh,U Hahn,H Wagner,J (1997). Efficacy of different methods for detection of low Cryptosporidium parvum oocyst numbers or antigen concentrations in stool specimens.
Stephanie Johnston,Melissa Ballard,Michael Beach,Louise Causer,Patricia Wilkins (2003). Evaluation of Three Commercial Assays for Detection of Giardia and Cryptosporidium Organisms in Fecal Specimens.
G Kennedy,G Kreitner,A Strafuss (1977). Cryptosporidiosis in three pigs.
Y Kuhnert-Paul,B Berit,D Katja,D Arwid,S Ronald (2012). Cryptosporidiosis: comparison of three diagnostic methods and effects of storage temperature on detectability of cryptosporidia in cattle faeces.
M Kvac,B Sak,D Hanzlikova,J Kotilova,D Kvetonova (2009). Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic.
J Kwaga,J Umoh,M Odoba (1988). Cryptosporidiuminfections in humans with gastroenteritis in Zaria, Nigeria.
N Mahdi,N Ali (2004). Cryptosporidiosis and other intestinal parasitic infections in patients with chronic diarrhoea.
B Maikai,J Umoh,J Kwaga,V Maikai,S Egege (2011). Prevalence and risk factors associated with faecal shedding of Cryptosporidium oocysts in piglets, Kaduna, Nigeria.
B Ungar (1990). Enzyme-linked immunoassay for detection of Cryptosporidium antigens in fecal specimens.
Rongjun Wang,Shuxing Qiu,Fuchun Jian,Sumei Zhang,Yujuan Shen,Longxian Zhang,Changshen Ning,Jianping Cao,Meng Qi,Lihua Xiao (2010). Prevalence and molecular identification of Cryptosporidium spp. in pigs in Henan, China.
L Xiao,R Fayer (2008). Molecular characterisation of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmission.
S Yatswako,O Faleke,M Gulumbe,A Daneji (2007). Cryptosporidium oocysts and Balantidium coli Cysts in Pigs Reared Semi-intensively in Zuru, Nigeria.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Akinkuotu Olufemi Ambrose, Jacobs Eniope Bamidele, Okwelum Ngozi. 2015. "Comparison of Two Methods in the Detection of Cryptosporidium in Pigs in Ogun State, Nigeria". Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 15 (GJMR Volume 15 Issue G1).

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GJMR Volume 15 Issue G1

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-G Classification NLMC Code: WC 900

Submission ReceivedDecember 13, 2014
Peer Review Double Blind
Handling Editor
Accepted January 2, 2015
Published January 15, 2015

Version of record

v1.2

Issue date

April 2, 2015

Language

English

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Total Score: 143

Country: Nigeria

Subject: Global Journal of Medical Research

Authors: Akinkuotu Olufemi Ambrose, Jacobs Eniope Bamidele, Okwelum Ngozi (PhD/Dr. count: 0)

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