Copper (II) Oxide Nanoparticles Induce High Toxicity in Human Neuronal Cell

Copper (II) Oxide Nanoparticles Induce High Toxicity in Human Neuronal Cell

Guzel E. Elif

Contact

Abudayyak Mahmoud

Contact

Ozhan GAl

Contact

Istanbul University

Copper (II) Oxide Nanoparticles Induce High Toxicity in Human Neuronal Cell

Article Fingerprint

ReserarchID

PDDTMC72T8

Copper (II) Oxide Nanoparticles Induce High Toxicity in Human Neuronal Cell Banner

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

Copper (II) oxide nanoparticles (CuO-NPs) are widely used in industry, cosmetics and medicine. People have increasingly been exposed to these active materials. Several studies indicate that CuO-NPs could be taken up by different organs and cause toxicities. However, there is still a lack of data on the toxicological effects of CuO-NPs in neuronal system. In the present study, the toxic potentials of CuO-NPs were investigated in human SH-SY5Y neuroblastoma cells. After assessment of their cellular uptake potential, cytotoxicity by MTT and neutral red uptake (NRU) and genotoxicity by comet assay were evaluated. Enzyme-Linked Immune Sorbent Assays (ELISA) determination of malondialdehyde (MDA), 8-hydroxydeoxyguanosine (8-OHdG), protein carbonyl (PC), and glutathione (GSH) levels for oxidative damage, and Annexin V-FITC with propidium iodide (PI) for apoptosis were used. In conclusion, CuO-NPs were found to accumulate in the cells and induced significant cytotoxic and genotoxic, and oxidative and apoptotic effects. CuO-NPs are hypothesized to dangerously affect human health, especially neuronal system.

References

38 Cites in Article

Reference Format

M Ahamed,D Ali,H Alhadlaq (2013). Nickel oxide nanoparticles exert cytotoxicity via oxidative stress and induce apoptotic response in human liver cells (HepG2).
M Abudayyak,T Altıncekic,G Özhan (2016). In vitro toxicological evaluation of cobalt ferrite nanoparticles.
Mahmoud Abudayyak,E Guzel,Gül Özhan (2016). Copper (II) Oxide Nanoparticles Induced Nephrotoxicity In Vitro Conditions.
Maqusood Ahamed,Maqsood Siddiqui,Mohd Akhtar,Iqbal Ahmad,Aditya Pant,Hisham Alhadlaq (2010). Genotoxic potential of copper oxide nanoparticles in human lung epithelial cells.
M Akhtar,M Ahamed,M Fareed (2012). Protective effect of sulphoraphane against oxidative stress mediated toxicity induced by CuO nanoparticles in mouse embryonic fibroblasts BALB 3T3.
Mohammad Akhtar,Sudhir Kumar,Hisham Alhadlaq,Salman Alrokayan,Khalid Abu-Salah,Maqusood Ahamed (2016). Dose-dependent genotoxicity of copper oxide nanoparticles stimulated by reactive oxygen species in human lung epithelial cells.
Saud Alarifi,Daoud Ali,Ankit Verma,Saad Alakhtani,Bahy Ali (2013). Cytotoxicity and Genotoxicity of Copper Oxide Nanoparticles in Human Skin Keratinocytes Cells.
L An,S Liu,Z Yang (2012). Cognitive impairment in rats induced by nano-CuO and its possible mechanisms.
D Barceloux (1999). Copper.
Marion Bradford (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding.
Ya-Nan Chang,Mingyi Zhang,Lin Xia,Jun Zhang,Gengmei Xing (2012). The Toxic Effects and Mechanisms of CuO and ZnO Nanoparticles.
Jian Chen,Jinmin Zhu,Hyun-Hee Cho,Kemi Cui,Fuhai Li,Xiaobo Zhou,Jack Rogers,Stephen Wong,Xudong Huang (2008). Differential cytotoxicity of metal oxide nanoparticles.
Wan-Seob Cho,Rodger Duffin,Craig Poland,Albert Duschl,Gertie Oostingh,William Macnee,Mark Bradley,Ian Megson,Ken Donaldson (2012). Differential pro-inflammatory effects of metal oxide nanoparticles and their soluble ions in vitro and in vivo ; zinc and copper nanoparticles, but not their ions, recruit eosinophils to the lungs.
A Collins (2004). The comet assay for DNA damage and repair principles, applications, and limitations.
P Cronholm,K Midander,H Karlsson (2011). Effect of sonication and serum proteins on copper release from copper nanoparticles and the toxicity towards lung epithelial cells.
M Cuillel,M Chevallet,P Charbonnier (2014). Interference of CuO nanoparticles with metal homeostasis in hepatocytes under sub-toxic conditions.
Andreas Elsaesser,C Howard (2011). Toxicology of nanoparticles.
Baher Fahmy,Stephania Cormier (2009). Copper oxide nanoparticles induce oxidative stress and cytotoxicity in airway epithelial cells.
Robert Griffitt,Roxana Weil,Kelly Hyndman,Nancy Denslow,Kevin Powers,David Taylor,David Barber (2007). Exposure to Copper Nanoparticles Causes Gill Injury and Acute Lethality in Zebrafish (Danio rerio).
Y Huang,C Wu,R Aronstam (2010). Toxicity of transition metal oxide nanoparticles: Recent insights from in vitro studies.
H Karlsson,P Cronholm,J Gustafsson (2008). Copper oxide nanoparticles are highly toxic: a comparison between metal oxide nanoparticles and carbon nanotubes.
G Kilic,C Costa,N Fernández-Bertólez (2016). In vitro toxicity evaluation of silica-coated iron oxide nanoparticles in human SHSY5Y neuronal cells.
Youn-Jung Kim,Miri Yu,Hee-Ok Park,Sung Yang (2010). Comparative study of cytotoxicity, oxidative stress and genotoxicity induced by silica nanomaterials in human neuronal cell line.
Dipranjan Laha,Arindam Pramanik,Jyotirindra Maity,Ananda Mukherjee,Panchanan Pramanik,Aparna Laskar,Parimal Karmakar (2014). Interplay between autophagy and apoptosis mediated by copper oxide nanoparticles in human breast cancer cells MCF7.
R Lei,C Wu,B Yang (2008). Integrated metabolomic analysis of the nano-sized copper particle-induced hepatotoxicity and nephrotoxicity in rats: a rapid in vivo screening method for nanotoxicity.
P Manna,M Ghosh,J Ghosh (2012). Contribution of nano-copper particles to in vivo liver dysfunction and cellular damage: role of IκBα/NF-κB, MAPKs and mitochondrial signal.
F Perreault,S Melegari,C De Costa (2012). Genotoxic effects of copper oxide nanoparticles in Neuro 2A cell cultures.
Jean-Pascal Piret,Diane Jacques,Jean-Nicolas Audinot,Jorge Mejia,Emmanuelle Boilan,Florence Noël,Maude Fransolet,Catherine Demazy,Stéphane Lucas,Christelle Saout,Olivier Toussaint (2012). Copper(ii) oxide nanoparticles penetrate into HepG2 cells, exert cytotoxicity via oxidative stress and induce pro-inflammatory response.
G Repetto,A Del Peso,J Zurita (2008). Neutral red uptake assay for the estimation of cell viability/ cytotoxicity.
M Siddiqui,H Alhadlaq,J Ahmad (2013). Copper oxide nanoparticles induced mitochondria mediated apoptosis in human hepatocarcinoma cells.
Günter Speit,Andreas Hartmann (1999). The Comet Assay (Single-Cell Gel Test): A Sensitive Genotoxicity Test for the Detection of DNA Damage and Repair.
J Sun,S Wang,D Zhao (2011). Cytotoxicity, permeability, and inflammation of metal oxide nanoparticles in human cardiac microvascular endothelial cells.
V Valdiglesias,C Costa,V Sharma (2013). Comparative study on effects of two different types of titanium dioxide nanoparticles on human neuronal cells.
Johan Van Meerloo,Gertjan Kaspers,Jacqueline Cloos (2011). Cell Sensitivity Assays: The MTT Assay.
Y Wang,W Aker,H Hwang (2011). A study of the mechanism of in vitro cytotoxicity of metal oxide nanoparticles using catfish primary hepatocytes and human HepG2 cells.
Z Wang,N Li,J Zhao (2012). CuO nanoparticle interaction with human epithelial cells: cellular uptake, location, export, and genotoxicity.
Min-Kyeong Yeo,Misook Kang (2009). Effects of Cu x TiO y nanometer particles on biological toxicity during zebrafish embryogenesis.
(null). Figure 7: X-ray diffraction analysis..

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Guzel E. Elif. 2016. \u201cCopper (II) Oxide Nanoparticles Induce High Toxicity in Human Neuronal Cell\u201d. Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 16 (GJMR Volume 16 Issue B3).

More Citation Formats

Select Citation Style:

Download Citation

GJMR Volume 16 Issue B3

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

Not Found

Classification

GJMR-B Classification NLMC Code: QV 600

Submission ReceivedDecember 10, 2015
Peer Review Double Blind
Handling Editor
Accepted January 2, 2016
Published January 15, 2016

Version of record

v1.2

Issue date

October 13, 2016

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 103

Country: Turkey

Subject: Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine

Authors: Abudayyak Mahmoud, Guzel E. Elif, Ozhan GAl (PhD/Dr. count: 0)

View Count (all-time): 146

Total Views (Real + Logic): 3555

Total Downloads (simulated): 1845

Publish Date: 2016 10, Thu

Monthly Totals (Real + Logic):

Month 1: 38 views
Month 2: 19 views
Month 3: 55 views
Month 4: 18 views
Month 5: 43 views
Month 6: 29 views
Month 7: 41 views
Month 8: 34 views
Month 9: 42 views
Month 10: 17 views
Month 11: 42 views
Month 12: 22 views
Month 13: 25 views
Month 14: 30 views
Month 15: 28 views
Month 16: 28 views
Month 17: 21 views
Month 18: 17 views
Month 19: 30 views
Month 20: 14 views
Month 21: 46 views
Month 22: 18 views
Month 23: 24 views
Month 24: 30 views
Month 25: 33 views
Month 26: 26 views
Month 27: 31 views
Month 28: 23 views
Month 29: 33 views
Month 30: 25 views
Month 31: 21 views
Month 32: 22 views
Month 33: 33 views
Month 34: 23 views
Month 35: 36 views
Month 36: 44 views
Month 37: 23 views
Month 38: 30 views
Month 39: 23 views
Month 40: 31 views
Month 41: 45 views
Month 42: 20 views
Month 43: 24 views
Month 44: 23 views
Month 45: 36 views
Month 46: 30 views
Month 47: 14 views
Month 48: 25 views
Month 49: 25 views
Month 50: 40 views
Month 51: 29 views
Month 52: 14 views
Month 53: 35 views
Month 54: 24 views
Month 55: 14 views
Month 56: 38 views
Month 57: 33 views
Month 58: 36 views
Month 59: 43 views
Month 60: 40 views
Month 61: 37 views
Month 62: 24 views
Month 63: 38 views
Month 64: 34 views
Month 65: 22 views
Month 66: 42 views
Month 67: 31 views
Month 68: 48 views
Month 69: 27 views
Month 70: 12 views
Month 71: 41 views
Month 72: 21 views
Month 73: 36 views
Month 74: 39 views
Month 75: 47 views
Month 76: 29 views
Month 77: 31 views
Month 78: 19 views
Month 79: 45 views
Month 80: 34 views
Month 81: 30 views
Month 82: 43 views
Month 83: 28 views
Month 84: 35 views
Month 85: 39 views
Month 86: 29 views
Month 87: 29 views
Month 88: 29 views
Month 89: 43 views
Month 90: 21 views
Month 91: 28 views
Month 92: 23 views
Month 93: 17 views
Month 94: 24 views
Month 95: 16 views
Month 96: 27 views
Month 97: 34 views
Month 98: 23 views
Month 99: 28 views
Month 100: 31 views
Month 101: 15 views
Month 102: 35 views
Month 103: 22 views
Month 104: 28 views
Month 105: 26 views
Month 106: 34 views
Month 107: 36 views
Month 108: 21 views
Month 109: 29 views
Month 110: 11 views
Month 111: 34 views
Month 112: 44 views
Month 113: 42 views
Month 114: 36 views
Month 115: 53 views

Total Views: 3555

Total Downloads: 1845

2026 Trends

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]