Cytotoxic Effects, Phytochemical and GC/MS Analyses of Bosciasenegalensis.L Leave Extracts

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manal_a_ibrahim
manal_a_ibrahim
2
Manal A Ibrahi
Manal A Ibrahi
3
Adam Kh Musa
Adam Kh Musa
1 Omdurman Islamic University

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Boscia senegalensis leaves which were traditionally used to relief intestinal pain in Sudan were tested for secondary metabolites and cytotoxic effects. The result indicated a moderate presence of alkaloids, highly presence of tannins, weakly presence of flavonoids and steroids and negative results for saponin, phenolic compound and triterpens. Methanolic extract was tested for cytotoxicity against brine shrimp larvae. Remarkable cytotoxicity was revealed with high value equal to 1.975 µg/ml. Also the extract was subjected to separation by column chromatography technique, four fractions were obtained. The fractions tested for cytotoxicity against brine shrimp larvae again. F 1 , F 2 , F 3 and F 4 represented high values equal to 66.13. 11.07, 1.74 and 99.41 µg/ml respectively. F 3 with high cytotoxicity was chosen for gas chromatography / mass spectrometry analysis. Thirty-five compounds were not recorded in any previous work in available literature were obtained. The high cytotoxicity of this fraction due to presence of octadecenoic and n-hexadecenoic acid which are known to have anticancer activity.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

manal_a_ibrahim. 2021. \u201cCytotoxic Effects, Phytochemical and GC/MS Analyses of Bosciasenegalensis.L Leave Extracts\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 21 (GJSFR Volume 21 Issue C1): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 270499
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v1.2

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March 8, 2021

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English

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Boscia senegalensis leaves which were traditionally used to relief intestinal pain in Sudan were tested for secondary metabolites and cytotoxic effects. The result indicated a moderate presence of alkaloids, highly presence of tannins, weakly presence of flavonoids and steroids and negative results for saponin, phenolic compound and triterpens. Methanolic extract was tested for cytotoxicity against brine shrimp larvae. Remarkable cytotoxicity was revealed with high value equal to 1.975 µg/ml. Also the extract was subjected to separation by column chromatography technique, four fractions were obtained. The fractions tested for cytotoxicity against brine shrimp larvae again. F 1 , F 2 , F 3 and F 4 represented high values equal to 66.13. 11.07, 1.74 and 99.41 µg/ml respectively. F 3 with high cytotoxicity was chosen for gas chromatography / mass spectrometry analysis. Thirty-five compounds were not recorded in any previous work in available literature were obtained. The high cytotoxicity of this fraction due to presence of octadecenoic and n-hexadecenoic acid which are known to have anticancer activity.

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Cytotoxic Effects, Phytochemical and GC/MS Analyses of Bosciasenegalensis.L Leave Extracts

Manal A Ibrahi
Manal A Ibrahi
Adam Kh Musa
Adam Kh Musa

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