Degradation of Crude Oil by Bacteria: A Role for Plasmid-Borne Genes

Akpe, Azuka Romanus; Afe Omolola

Degradation of Crude Oil by Bacteria: A Role for Plasmid-Borne Genes

Akpe, Azuka Romanus * §

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Afe Omolola

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Ambrose Alli University

Degradation of Crude Oil by Bacteria: A Role for Plasmid-Borne Genes

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Abstract

The role of plasmid-borne genes in the biodegradation of Chevron Escravos Crude Oil by bacteria was determined. Plasmid extraction and curing, transformation experiments and biodegradation studies were carried out using standard procedures. Plasmid extraction studies showed that two of the six selected crude oil degrading bacterial isolates had two plasmids each. The isolates were Klebsiella pneumoniae from ripe pawpaw fruit and Serratia marscencens from oil palm mill effluent. The plasmids were of small (300bp) and large (>1.5kbp) sizes. The results also showed that the isolates were successfully cured of plasmids using 1% Sodium Dodecyl Sulphate (SDS). The transformation experiment using the extracted plasmid DNA and competent Escherichia coli K12 DH1 cells was successful. The percentage degradation of crude oil at 37oC by E. coli K12 DH1 transformed with the plasmid DNA from Klebsiella pneumoniae was 93.03% while that transformed with the plasmid DNA from Serratia marscencens degraded 76.97% of the crude oil. It was observed that loss of plasmids by Klebsiella pneumoniae and Serratia marscencens did not lead to complete loss of their degradative abilities. It only resulted in reduction in their degradation potential.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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How to Cite This Article

Akpe, Romanus, Afe Omolola. 2013. "Degradation of Crude Oil by Bacteria: A Role for Plasmid-Borne Genes". Global Journal of Science Frontier Research, Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 13 (GJSFR Volume 13 Issue C6).

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GJSFR Volume 13 Issue C6

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

bacterial isolates biodegradation crude oil plasmid curing plasmid DNA transformation

Classification

GJSFR-C Classification FOR Code: 279999p

Submission ReceivedAugust 15, 2013
Peer Review Double Blind
Handling Editor
Accepted September 14, 2013
Published September 29, 2013

Version of record

v1.2

Issue date

October 2, 2013

Language

English

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Article Matrices

Total Score: 150

Country: Nigeria

Subject: Global Journal of Science Frontier Research

Authors: Akpe, Azuka Romanus, Ekundayo, Afe Omolola, Esumeh, Frederick Ikechukwu (PhD/Dr. count: 0)

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