Neural Networks and Rules-based Systems used to Find Rational and Scientific Correlations between being Here and Now with Afterlife Conditions
February 17, 2026
Neural Networks and Rules-based Systems used to Find Rational and
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The design of mammalian cell culture processes as technological platform for monoclonal antibody (mAb) production is a complex task mainly due to partial knowledge of culture media composition impact on process outcomes. Faced with this problem, the present work aimed to characterize the metabolic profile during the early culture at lab-scale of a specific cell line transfected to obtain a monoclonal antibody (mAb) of therapeutic interest in the treatment of cancer, seeking most favorable nutritional conditions. The experimental design, based on the use of four different media in a two-liter scale culture, provided data on the content of 19 metabolites, cell concentration, and mAb concentration over the course of batches, where in the first case measurements were performed with liquid chromatography-mass spectrometry (LC-MS) as an advanced laboratory analytical support.
Erick Hernández. 2026. \u201cDetermination of Best Nutritional Conditions for a Monoclonal Antibody-Producing Cell Line based on a Multivariate Data Analysis Approach\u201d. Global Journal of Research in Engineering - J: General Engineering GJRE-J Volume 23 (GJRE Volume 23 Issue J1): .
Crossref Journal DOI 10.17406/gjre
Print ISSN 0975-5861
e-ISSN 2249-4596
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The design of mammalian cell culture processes as technological platform for monoclonal antibody (mAb) production is a complex task mainly due to partial knowledge of culture media composition impact on process outcomes. Faced with this problem, the present work aimed to characterize the metabolic profile during the early culture at lab-scale of a specific cell line transfected to obtain a monoclonal antibody (mAb) of therapeutic interest in the treatment of cancer, seeking most favorable nutritional conditions. The experimental design, based on the use of four different media in a two-liter scale culture, provided data on the content of 19 metabolites, cell concentration, and mAb concentration over the course of batches, where in the first case measurements were performed with liquid chromatography-mass spectrometry (LC-MS) as an advanced laboratory analytical support.
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