Development and Validation of Derivative FTIR Spectroscopy for Estimation of Entecavir Monohydrate in its Pure and Pharmaceutical Dosage Forms

1
Ashraf A Khanam
Ashraf A Khanam
2
Y Padmavathi
Y Padmavathi
3
Raghavendra Babu
Raghavendra Babu
1 G. Pulla Reddy College of Pharmacy, Osmania University

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We developed a unique analytical technique for the evaluation of Entecavir monohydrate (ETV) in its pharmaceutical dosage form using derivative spectroscopy assisted FTIR. This approach requires the formation of solid pellets of Entecavir using potassium bromide (KBr) with the aid of geometrical mixing. The spectra were calculated by direct measurement technique using reduced path length in the absorbance mode, and the equipment was configured to secure it at 8cm -1 resolution. We scanned the spectra between the ranges of 4000 to 400 cm -1 . FTIR spectra drug exhibited overlapped functional group peaks with baseline correction at 1631 cm -1 corresponding to C=O stretching. From these FTIR spectra, we detected intense, clear, and proportional second derivative peaks between 1639.38 and 1620.09 cm -1 . These peaks, in the range of concentration 12.5-200 μg/mg, obeyed Beer-Lambert’s law. Therefore, we elected C=O stretching for the quantitative evaluation of Entecavir employing second-order derivative spectroscopy.

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No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Ashraf A Khanam. 2020. \u201cDevelopment and Validation of Derivative FTIR Spectroscopy for Estimation of Entecavir Monohydrate in its Pure and Pharmaceutical Dosage Forms\u201d. Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 20 (GJMR Volume 20 Issue B5): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-B Classification: NLMC Code: QV 701
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July 27, 2020

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English

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We developed a unique analytical technique for the evaluation of Entecavir monohydrate (ETV) in its pharmaceutical dosage form using derivative spectroscopy assisted FTIR. This approach requires the formation of solid pellets of Entecavir using potassium bromide (KBr) with the aid of geometrical mixing. The spectra were calculated by direct measurement technique using reduced path length in the absorbance mode, and the equipment was configured to secure it at 8cm -1 resolution. We scanned the spectra between the ranges of 4000 to 400 cm -1 . FTIR spectra drug exhibited overlapped functional group peaks with baseline correction at 1631 cm -1 corresponding to C=O stretching. From these FTIR spectra, we detected intense, clear, and proportional second derivative peaks between 1639.38 and 1620.09 cm -1 . These peaks, in the range of concentration 12.5-200 μg/mg, obeyed Beer-Lambert’s law. Therefore, we elected C=O stretching for the quantitative evaluation of Entecavir employing second-order derivative spectroscopy.

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Development and Validation of Derivative FTIR Spectroscopy for Estimation of Entecavir Monohydrate in its Pure and Pharmaceutical Dosage Forms

Ashraf A Khanam
Ashraf A Khanam G. Pulla Reddy College of Pharmacy, Osmania University
Y Padmavathi
Y Padmavathi
Raghavendra Babu
Raghavendra Babu

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