DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

1
Chiedozie Joel Uka
Chiedozie Joel Uka
2
Daniel Azubuike Awomukwu
Daniel Azubuike Awomukwu
3
Bio Louis Nyananyo
Bio Louis Nyananyo
4
Paula Spies
Paula Spies
5
Bulelani Londoloza Sizani
Bulelani Londoloza Sizani
1 Federal University
2 Federal University, Otueke, Bayelsa State, Nigeria

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Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

31 Cites in Articles

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Chiedozie Joel Uka. 2015. \u201cDNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 15 (GJSFR Volume 15 Issue G1): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-G Classification: 069999
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October 17, 2015

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English

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Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

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DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

Daniel Azubuike Awomukwu
Daniel Azubuike Awomukwu Federal University, Otueke, Bayelsa State, Nigeria
Bio Louis Nyananyo
Bio Louis Nyananyo
Chiedozie Joel Uka
Chiedozie Joel Uka Federal University
Paula Spies
Paula Spies
Bulelani Londoloza Sizani
Bulelani Londoloza Sizani

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