DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

Chiedozie Joel Uka

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Daniel Azubuike Awomukwu

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Bio Louis Nyananyo

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Paula Spies

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Bulelani Londoloza Sizani

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α Federal University

σ Federal University Otuoke

DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

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Abstract

Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Chiedozie Joel Uka. 2015. \u201cDNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers\u201d. Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 15 (GJSFR Volume 15 Issue G1): .

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GJSFR Volume 15 Issue G1
Pg. 1- 12

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-G Classification: 069999

Submission ReceivedApril 27, 2015
Peer Review Double Blind
Handling Editor
Accepted May 25, 2015
Published June 7, 2015

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v1.2

Issue date

October 17, 2015

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Article Matrices

Total Score: 105

Country: Nigeria

Subject: Global Journal of Science Frontier Research - G: Bio-Tech & Genetics

Authors: Daniel Azubuike Awomukwu, Bio Louis Nyananyo, Chiedozie Joel Uka, Paula Spies, Bulelani Londoloza Sizani (PhD/Dr. count: 0)

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Total Views: 4081

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