DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

Chiedozie Joel Uka; Daniel Awomukwu; Bio Louis Nyananyo

DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

Chiedozie Joel Uka * §

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Daniel Azubuike Awomukwu

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Bio Louis Nyananyo

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§ Federal University

DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers

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Abstract

Mull-Arg. and P. muellerianus (O. Ktze) Excel belonging to the family of Phyllanthaceae were extracted, purified, PCR amplified and sequenced using plastid Ribulose -1,5 bisphosphate carboxylase large chain (rbcL a) and Maturase K (matK) genetic marker to identify unknown Phyllanthus species. The plastid region revealed that the Phyllanthus species were able to be amplified optimally for sequencing. The results of the nucleotide sequences were further compared on Basic Local Alignment Sequence Tool (BLAST) on Gen Bank and Bold Systems for validation. Results revealed that the closely related species, P. niruroides Mull_Arg. and P. odontadenius Mull-Arg. had no DNA record to separate them on both GenBank and Bold Systems using both rbcL and matK gene regions while P. amarus Schum and Thonn and P. urinaria Linn. were clearly compatible with other works. P. muellerianus (O. Ktze) Excel was only compatible with other works using matK gene region but none for rbcL.

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31 Cites in Article

Reference Format

D Awomukwu,B Nyananyo,N Onukwube,C Uka,C Okeke,A Ikpeama (2014). Comparative Phytochemical Constituents and Pharmacognistic Importance of the Vegetative Organs of Some Phyllanthus species in South Eastern Nigeria.
D Awomukwu,B Nyananyo,P Spies,B Sizani (2015). Identification, Validation and Classification of the genus Phyllanthus in Nigeria using ITS Genetic Marker and the Taxonomic Implication.
L Bancilhon (1971). Contribution a´ l'e´tude taxonomique du genre Phyllanthus (Euphorbiace´es).
M Blaxter (2004). The promise of a DNA taxonomy.
Peter Hollingsworth (2009). Refining the DNA barcode for land plants.
J Doyle,J Doyle (1987). A rapid DNA isolation procedure for small quantities of fresh leaf tissue.
J Doyle,J Doyle (1990). Isolation of plant DNA from fresh tissue.
H Edeoga,G Omosun,G Osuagwu,D Awomukwu (2007). Anatomical Features of Vegetative Organs of Phyllanthus species.
R Henry (2001). Plant genotyping: The DNA fingerprinting of plants.
J Hutchinson,M Dalziel (1963). Flora of West Tropical Africa.
H Kathriarachchi,P Hoffmann,R Samuel,K Wurdack,M Chase (2005). Molecular phylogenetics of Phyllanthaceae inferred from five genes (plastid atpB, matK, 30ndhF, rbcL, and nuclear PHYC).
W Kress,D Erickson (2007). A two-locus global DNA barcode for land plants: the coding rbcL gene complements the non-coding trnH-psbA spacer region.
W Kress,Kenneth Wurdack,Elizabeth Zimmer,Lee Weigt,Daniel Janzen (2005). Use of DNA barcodes to identify flowering plants.
E Ko¨ Hler (1965). Die Pollenmorphologie der biovulaten Euphorbiaceae und ihre Bedeutung fu¨ r die Taxonomie.
E Ko¨ Hler (1967). U¨ ber Beziehungen zwischen Pollenmorphologie and Polyploidiestufen im Verwandtschaftsbereich der Gattung Phyllanthus (Euphorbiaceae).
V Kumari,A Bansal,R Aminedi,D Taneja,N Das (2012). Simplified extraction of good quality genomic DNA from a variety of plant materials.
D Paul,A Herbert,L Shelly,R Jeremy (2003). Biological Identification through DNA barcodes.
W Punt (1967). Pollen morphology of the genus Phyllanthus (Euphorbiaceae).
W Punt (1987). A survey of pollen morphology in Euphorbiaceae with special reference to Phyllanthus.
M Rahman (2007). DNA fingerprinting in Utricularia L. Section Utricularia as revealed by PCR based assay.
V Sagun,R Van Der Ham (2003). Pollen morphology of the Flueggeinae (Euphorbiaceae, Phyllanthoideae).
Rosabelle Samuel,Hashendra Kathriarachchi,Petra Hoffmann,Michael Barfuss,Kenneth Wurdack,Charles Davis,Mark Chase (2005). Molecular phylogenetics of Phyllanthaceae: evidence from plastid MATK and nuclear PHYC sequences.
M Stoeckle,D Janzen,W Hallwachs,J Hanken,J Baker (2003). Taxonomy, DNA, and the Barcode of Life.
M Stoeckle,P Waggoner,J Ausubel (2004). Walcott, Charles Doolittle, (31 March 1850–9 Feb. 1927), Secretary, Smithsonian Institution, Washington, DC, since 1907.
N Sucher,M Charles (2008). Genome-Based approaches to the authentication of medicinal plants.
C Uka,C Okeke,D Awomukwu,B Aziagba,R Muoka (2014). Taxonomic Significance of Foliar Epidermis of some Phyllanthus species in South Eastern Nigeria.
G Webster (1956). A monographic study of the West Indian species of Phyllanthus.
G Webster,K Carpenter (2002). Pollen morphology and phylogenetic relationships in neotropical Phyllanthus (Euphorbiaceae).
Grady Webster,J Ellis (1962). CYTOTAXONOMIC STUDIES IN THE EUPHORBIACEAE, SUBTRIBE PHYLLANTHINAE.
K Weisling,H Nybom,K Wolff,G Kahl (2005). DNA fingerprinting in plants: Principles, methods and applications.
Kenneth Wurdack,Petra Hoffmann,Rosabelle Samuel,Anette De Bruijn,Michelle Van Der Bank,Mark Chase (2004). Molecular phylogenetic analysis of Phyllanthaceae (Phyllanthoideae pro parte, Euphorbiaceae sensu lato) using plastid <i>RBCL</i> DNA sequences.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Chiedozie Uka, Daniel Awomukwu, Bio Nyananyo, Chiedozie Uka. 2015. "DNA Barcoding, Identification and Validation of the Genus Phyllanthus in Nigeria using Plastid rbcL and matK Genetic Markers". Global Journal of Science Frontier Research, Global Journal of Science Frontier Research - G: Bio-Tech & Genetics GJSFR-G Volume 15 (GJSFR Volume 15 Issue G1).

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GJSFR Volume 15 Issue G1

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

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Classification

GJSFR-G Classification 069999

Submission ReceivedApril 27, 2015
Peer Review Double Blind
Handling Editor
Accepted May 25, 2015
Published June 7, 2015

Version of record

v1.2

Issue date

October 17, 2015

Language

English

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Article Matrices

Total Score: 149

Country: Nigeria

Subject: Global Journal of Science Frontier Research

Authors: Daniel Azubuike Awomukwu, Bio Louis Nyananyo, Chiedozie Joel Uka, Paula Spies, Bulelani Londoloza Sizani (PhD/Dr. count: 0)

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