Effect of Combinations of Mycobacteriophages and Drugs against Mycobacterium Spp.

Effect of Combinations of Mycobacteriophages and Drugs against Mycobacterium Spp.

Rajitha Satish

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Anita Desouza

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Effect of Combinations of Mycobacteriophages and Drugs against Mycobacterium Spp.

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Abstract

The emergence of multidrug-resistant tuberculosis (MDR-TB) is a matter of global concern. The use of mycobacteriophages alone or in combination with antibiotics could be used as an alternative approach to treat drug-resistant Mycobacteria. The aim of the study was the evaluation of the effect of an isolated mycobacteriophage in combination with Isoniazid, Rifampicin, Streptomycin, and Ampicillin against Mycobacterium tuberculosis and Mycobacterium smegmatis. Determination of the MIC of the above drugs for each strain was done by the standard tube dilution method. Further, the inhibitory effect of varying drug concentrations in combination with different dilutions of the mycobacteriophage was studied. The growth inhibition pattern for both the organisms was also studied in the presence of different dilutions of mycobacteriophage by turbidometric and resazurin dye reduction method. The killing pattern of drugs, at concentrations below the MIC, when combined with mycobacteriophage, was determined. The phage induced lysis of bacteria assisted in decreasing the inhibitory concentration of the drugs.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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How to Cite This Article

Rajitha Satish. 2020. \u201cEffect of Combinations of Mycobacteriophages and Drugs against Mycobacterium Spp.\u201d. Global Journal of Science Frontier Research - B: Chemistry GJSFR-B Volume 19 (GJSFR Volume 19 Issue B3).

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GJSFR Volume 19 Issue B3

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-B Classification FOR Code: 030499

Submission ReceivedOctober 6, 2019
Peer Review Double Blind
Handling Editor
Accepted November 4, 2019
Published November 16, 2019

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January 10, 2020

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