Effect of Cryopreservation on Biological Markers of Sperm Function and their Correlation in Different Dog Breeds

Effect of Cryopreservation on Biological Markers of Sperm Function and their Correlation in Different Dog Breeds

Pathania A

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Ranjna S Cheema

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Sharma A

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Guru Angad Dev Veterinary and Animal Sciences University

Effect of Cryopreservation on Biological Markers of Sperm Function and their Correlation in Different Dog Breeds

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Abstract

In the present study, we compared motility, viability, plasma membrane integrity (PMI), acrosome integrity (AI), inner mitochondrial membrane potential (IMMP), ATP and LDHC in freshly extended (FE,) and frozen-thaw (FT) semen among the dogs and breeds. Freshly extended semen in Tris-citric acid-fructose-egg yolk plasma-glycerol extenderwas also subjected to in vitro capacitation and acrosome reaction. Pearson correlation coefficient among the sperm attributes of FE and FT semen has been evaluated.Sperm attributes in FE and FT semen vary significantly (P

References

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S Abedin,V Leela,P Devendran,G Suganya,S Rangasamy,K Loganathasamy (2020). Seminal Plasma Osteopontin: A Marker for Potential Fertility in Dogs.
J Albarracín,T Mogas,M Palomo,A Peña,T Rigau,J Rodríguez‐gil (2004). <i>In vitro</i> Capacitation and Acrosome Reaction of Dog Spermatozoa can be Feasibly Attained in a Defined Medium Without Glucose.
Falah Baiee,Haron Wahid,Yusoff Rosnina,Omar Ariff,Nurhusien Yimer,Zaid Jeber,Hammadi Salman,Ahmedeltayeb Tarig,Fazel Harighi (2018). Impact of Eurycoma longifolia extract on DNA integrity, lipid peroxidation, and functional parameters in chilled and cryopreserved bull sperm.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Pathania A. 2021. \u201cEffect of Cryopreservation on Biological Markers of Sperm Function and their Correlation in Different Dog Breeds\u201d. Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 21 (GJMR Volume 21 Issue G1).

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GJMR Volume 21 Issue G1

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-G Classification NLMC Code: QW 70

Submission ReceivedApril 8, 2021
Peer Review Double Blind
Handling Editor
Accepted May 1, 2021
Published May 15, 2021

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v1.2

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July 13, 2021

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