Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

1
Barakat Ilham
Barakat Ilham
2
Chtaina Noureddine
Chtaina Noureddine
3
Grappin Philippe
Grappin Philippe
4
El Guilli Mohemmed
El Guilli Mohemmed
5
Abdenbi El Hassane
Abdenbi El Hassane
6
Ezzahiri Brahim
Ezzahiri Brahim
1 Institute of Agronomic and Veterinary Medicine

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The strain I3 of Bacillus amyloliquefaciens, which was isolated from soft wheat leaves, has revealed, in vitro and in vivo, a high antagonistic potential against septoria leaf blotch of wheat. In order to investigate the existence of antifungal molecules secreted by strain I3, the filtrates of this strain were tested for their inhibitory activity on the germination of pycnidiospores of the two strains of Zymoseptoria tritici, G1-1 and A5-1 isolated from soft wheat and durum wheat, respectively. The antibiosis assays showed a high level of inhibitory activity, with inhibition rates ranging from 94% to 99% compared to the control after 96 hours of incubation. Filtrate analysis by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), have identified several families of lipopeptides reported as antifungal molecules (iturins, fengycins, and surfactins); and polyketides (macrolactins, chlorotetaines, and bacillaenes) which would also be responsible for the antagonistic activity against Z. tritici.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Barakat Ilham. 2020. \u201cEfficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 20 (GJSFR Volume 20 Issue D1): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-D Classification: FOR Code: 070199
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v1.2

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January 30, 2020

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English

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The strain I3 of Bacillus amyloliquefaciens, which was isolated from soft wheat leaves, has revealed, in vitro and in vivo, a high antagonistic potential against septoria leaf blotch of wheat. In order to investigate the existence of antifungal molecules secreted by strain I3, the filtrates of this strain were tested for their inhibitory activity on the germination of pycnidiospores of the two strains of Zymoseptoria tritici, G1-1 and A5-1 isolated from soft wheat and durum wheat, respectively. The antibiosis assays showed a high level of inhibitory activity, with inhibition rates ranging from 94% to 99% compared to the control after 96 hours of incubation. Filtrate analysis by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), have identified several families of lipopeptides reported as antifungal molecules (iturins, fengycins, and surfactins); and polyketides (macrolactins, chlorotetaines, and bacillaenes) which would also be responsible for the antagonistic activity against Z. tritici.

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Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

Barakat Ilham
Barakat Ilham Institute of Agronomic and Veterinary Medicine
Chtaina Noureddine
Chtaina Noureddine
Grappin Philippe
Grappin Philippe
El Guilli Mohemmed
El Guilli Mohemmed
Abdenbi El Hassane
Abdenbi El Hassane
Ezzahiri Brahim
Ezzahiri Brahim

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