Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

Barakat Ilham; Chtaina Noureddine; Grappin Philippe; El Guilli Mohemmed; Abdenbi El Hassane; Ezzahiri Brahim

Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

Barakat Ilham * §

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Chtaina Noureddine §

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Grappin Philippe §

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El Guilli Mohemmed §

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Abdenbi El Hassane §

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Ezzahiri Brahim §

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§ Institute of Agronomic and Veterinary Medicine

Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici

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Abstract

The strain I3 of Bacillus amyloliquefaciens, which was isolated from soft wheat leaves, has revealed, in vitro and in vivo, a high antagonistic potential against septoria leaf blotch of wheat. In order to investigate the existence of antifungal molecules secreted by strain I3, the filtrates of this strain were tested for their inhibitory activity on the germination of pycnidiospores of the two strains of Zymoseptoria tritici, G1-1 and A5-1 isolated from soft wheat and durum wheat, respectively. The antibiosis assays showed a high level of inhibitory activity, with inhibition rates ranging from 94% to 99% compared to the control after 96 hours of incubation. Filtrate analysis by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS), have identified several families of lipopeptides reported as antifungal molecules (iturins, fengycins, and surfactins); and polyketides (macrolactins, chlorotetaines, and bacillaenes) which would also be responsible for the antagonistic activity against Z. tritici.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

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How to Cite This Article

Barakat Ilham, Chtaina Noureddine, Grappin Philippe, El Guilli Mohemmed, Abdenbi El Hassane, Ezzahiri Brahim. 2020. "Efficacy of Secondary Metabolites Produced by Bacillus Amyloliquefaciens on the Inhibition of Zymoseptoria Tritici". Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 20 (GJSFR Volume 20 Issue D1).

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-D Classification FOR Code: 070199

Submission ReceivedNovember 24, 2019
Peer Review Double Blind
Handling Editor
Accepted December 22, 2019
Published January 30, 2020

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v1.2

Issue date

January 30, 2020

Language

English

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Total Score: 126

Country: Morocco

Subject: Global Journal of Science Frontier Research - D: Agriculture & Veterinary

Authors: Barakat Ilham, Chtaina Noureddine, Grappin Philippe, El Guilli Mohemmed, Abdenbi El Hassane, Ezzahiri Brahim (PhD/Dr. count: 0)

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