Efficient Production of Goat and Sheep Embryos under Similar in Vitro Conditions

1
Jose Carlos Ferreira-Silva
Jose Carlos Ferreira-Silva
2
Marcelo Tigre Moura
Marcelo Tigre Moura
3
Pabola Santos Nascimento
Pabola Santos Nascimento
4
Pamela Ramos-Deus
Pamela Ramos-Deus
5
Maiana Silva Chaves
Maiana Silva Chaves
6
Luis Rennan Sampaio Oliveira
Luis Rennan Sampaio Oliveira
7
Marcos Antonio Lemos Oliveira
Marcos Antonio Lemos Oliveira
1 Universidade Federal Rural de Pernambuco

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In vitro embryo production (IVP) protocols often require species-specific adaptations, in order to obtain embryonic development at high rates. However, these differences in IVP protocols make interspecific comparisons more susceptible to technical factors. The work aimed to compare IVP embryo production in small ruminants (e.g. goats and sheep) using identical in vitro oocyte maturation and fertilization. Oocytes were retrieved, matured, and fertilized under identical media and atmosphere conditions, while embryos were cultured under different conditions. Goat embryos were cultured in KSOM medium under co-culture, while sheep embryos were cultured in SOF medium under low oxygen tension without feeder cells. Cleavage, morula, and blastocyst development were similar for both goat and sheep, suggesting that oocyte maturation and fertilization conditions allowed a similar developmental potential for small ruminant species.

24 Cites in Articles

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

Jose Carlos Ferreira-Silva. 2017. \u201cEfficient Production of Goat and Sheep Embryos under Similar in Vitro Conditions\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D4): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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September 11, 2017

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In vitro embryo production (IVP) protocols often require species-specific adaptations, in order to obtain embryonic development at high rates. However, these differences in IVP protocols make interspecific comparisons more susceptible to technical factors. The work aimed to compare IVP embryo production in small ruminants (e.g. goats and sheep) using identical in vitro oocyte maturation and fertilization. Oocytes were retrieved, matured, and fertilized under identical media and atmosphere conditions, while embryos were cultured under different conditions. Goat embryos were cultured in KSOM medium under co-culture, while sheep embryos were cultured in SOF medium under low oxygen tension without feeder cells. Cleavage, morula, and blastocyst development were similar for both goat and sheep, suggesting that oocyte maturation and fertilization conditions allowed a similar developmental potential for small ruminant species.

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Efficient Production of Goat and Sheep Embryos under Similar in Vitro Conditions

Jose Carlos Ferreira-Silva
Jose Carlos Ferreira-Silva Universidade Federal Rural de Pernambuco
Marcelo Tigre Moura
Marcelo Tigre Moura
Pabola Santos Nascimento
Pabola Santos Nascimento
Pamela Ramos-Deus
Pamela Ramos-Deus
Maiana Silva Chaves
Maiana Silva Chaves
Luis Rennan Sampaio Oliveira
Luis Rennan Sampaio Oliveira
Marcos Antonio Lemos Oliveira
Marcos Antonio Lemos Oliveira

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