Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

Dr. Gitali Bhagawati

Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

Dr. Gitali Bhagawati *

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Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

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Abstract

Staphylococcus aureus is one of the major resistant pathogens in clinical practice; Methicillin Resistant Staphylococcus aureus (MRSA) has come out as superbugs. Apart from this, with the increase in the number of hospitalized immunocompromised patients, Coagulase negative Staphylococcus (CONS) have become a major cause of nosocomial infections. Although molecular method like mecA gene detection is gold standard for MRSA, minimum inhibitory concentration (MIC) of cefoxitin or oxacillin can also be considered as standard where molecular methods are not available. Cefoxitin 30 μg disc or PBP 2a agglutination test can also be used as standard marker for MRSA identification. In this study, out of total 184 clinically significant, non-duplicate specimens, 150 (81.52%) isolates were Staphylococcus aureus and 34 (18.48%) were CONS. Among the CONS, the predominating isolate was Staphylococcus haemolyticus 15 (44.12%), followed by Staphylococcus epidermidis 10 (29.41%). In our study, cefoxitin disk diffusion test was found to have sensitivity 100%, specificity 92.15% and negative predictive value (NPV) 100%. PBP2a latex agglutination test was found to have sensitivity 99%, specificity 97.87% and negative predictive value (NPV) 97.87% in our study. In both the methods, MIC of cefoxitin has been considered as gold standard.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Dr. Gitali Bhagawati. 2020. "Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus". Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 20 (GJMR Volume 20 Issue C4).

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GJMR Volume 20 Issue C4

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

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Classification

GJMR-C Classification NLMC Code: WQ 4

Submission ReceivedDecember 8, 2019
Peer Review Double Blind
Handling Editor
Accepted January 2, 2020
Published January 15, 2020

Version of record

v1.2

Issue date

October 12, 2020

Language

English

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