Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

Dr. Gitali Bhagawati

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Epidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus

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Abstract

Staphylococcus aureus is one of the major resistant pathogens in clinical practice; Methicillin Resistant Staphylococcus aureus (MRSA) has come out as superbugs. Apart from this, with the increase in the number of hospitalized immunocompromised patients, Coagulase negative Staphylococcus (CONS) have become a major cause of nosocomial infections. Although molecular method like mecA gene detection is gold standard for MRSA, minimum inhibitory concentration (MIC) of cefoxitin or oxacillin can also be considered as standard where molecular methods are not available. Cefoxitin 30 μg disc or PBP 2a agglutination test can also be used as standard marker for MRSA identification. In this study, out of total 184 clinically significant, non-duplicate specimens, 150 (81.52%) isolates were Staphylococcus aureus and 34 (18.48%) were CONS. Among the CONS, the predominating isolate was Staphylococcus haemolyticus 15 (44.12%), followed by Staphylococcus epidermidis 10 (29.41%). In our study, cefoxitin disk diffusion test was found to have sensitivity 100%, specificity 92.15% and negative predictive value (NPV) 100%. PBP2a latex agglutination test was found to have sensitivity 99%, specificity 97.87% and negative predictive value (NPV) 97.87% in our study. In both the methods, MIC of cefoxitin has been considered as gold standard.

References

20 Cites in Article

Reference Format

Manju Pillai,Ragunathan Latha,Gautam Sarkar (2012). Detection of Methicillin Resistance in Staphylococcus Aureus by Polymerase Chain Reaction and Conventional Methods: A Comparative Study.
Kennedy Stephen T Odonkora,Addob (2011). Evaluation of Three Methods for Detection of Methicillin Resistant Staphylococcus aureus (MRSA).
M Mimica,E Berezin,R Carvalho,I Mimica,L Mimica,M Sáfadi,E Schneider,H Caiaffa-Filho (2007). Detection of methicillin resistance in Staphylococcus aureus isolated from pediatric patients: is the cefoxitin disk diffusion test accurate enough?.
R Kaur,L Oberoi,A Aggarwal (2012). Comparative evaluation of Latex agglutination method with other phenotypic methods for detection of Methicillin-resistant Staphylococcus aureus.
Y Surekha,Asangi,Sathyanarayan Mariraj,Rashmi Nagabhushan (2011). Speciation of clinically significant Coagulase Negative Staphylococci and their antibiotic resistant patterns in a tertiary care hospital.
(2006). Performance standards for antimicrobial susceptibility testing.
Mohammad Zeeshan,Erum Kausarjabeen,Seema Khan,Saira Irfan,Ibrahim,Afiazafar Zebaparween (2007). Comparison of Different Phenotypic Methods of Detection of Methicillin Resistance in Staphylococcus aureuswith The Molecular Detection of mec-A Gene.
(2015). M100-S11, Performance standards for antimicrobial susceptibility testing.
F Amilcar,Samuel Cardona,Wilson (2015). Skin and Soft-Tissue Infections: A Critical Review and the Role of Telavancin in Their Treatment.
Anant Parasher,Pushpender Khatana (2020). Methicillin resistant Staphylococcus aureus: a review of the present Indian scenario and drug treatment.
Helio Sader,David Farrell,Ronald Jones (2010). Antimicrobial susceptibility of Gram-positive cocci isolated from skin and skin-structure infections in European medical centres.
Reinhard Berner,Siegfried Sauter,Ulrich Duffner,Matthias Brandis,Charlotte Niemeyer (1998). Bakteriämie-Episoden bei pädiatrisch-onkologischen Patienten, insbesondere durch Streptokokken der Viridans-Gruppe.
Gray James,Gossain Savita,P Morris (2001). Kevin Threeyear survey of bacteremia and fungemia in a pediatric intensive care unit.
Amita Jain,Astha Agarwal,Raj Verma,* Shallyawasthi,K Singh (2011). Intravenous device associated blood stream staphylococcal infection in paediatric patients.
H Robert,Cynthia Eng,Audrey Wang,Timothy Person,Donald Kiehn,Armstrong (1982). Species Identification of Coagulage-Negative Staphylococcal Isolates from Blood Cultures.
(2013). Indian Network for Surveillance of Antimicrobial Resistance (INSAR) group, India. Methicillin resistant Staphylococcus aureus (MRSA) in India: Prevalence & susceptibility pattern.
V Özel G 1 , Aslan,G Baharerdem,M Çağatay,I Sencan,A Mert (2011). Comparison of oxacillin, cefoxitin, ceftizoxime, and moxalactam disk diffusion methods for detection of methicillin susceptibility in staphylococci.
M Gupta,N Singh,A Kumar,I Kaur (2009). Cefoxitin Disk Diffusion Test - Better Predictor of Methicillin Resistance in Staphylococcus aureus.
Kennedy Stephen T Odonkor,Addo (2011). Evaluation of Three Methods For Detection of Methicillin Resistant Staphylococcus aureus (MRSA).
Elizabeth Marlowe,Andrea Linscott,Meganne Kanatani,David Bruckner (2002). Practical Therapeutic Application of the Oxoid PBP2’ Latex Agglutination Test for the Rapid Identification of Methicillin-Resistant<i>Staphylococcus aureus</i>in Blood Cultures.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Dr. Gitali Bhagawati. 2020. \u201cEpidemiology of Staphylococcus spp. with Analysis of Various Available Methods for Detection of Methicillin Resistant Staphylococcus Aureus\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 20 (GJMR Volume 20 Issue C4).

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GJMR Volume 20 Issue C4

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-C Classification NLMC Code: WQ 4

Submission ReceivedDecember 8, 2019
Peer Review Double Blind
Handling Editor
Accepted January 2, 2020
Published January 15, 2020

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v1.2

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October 12, 2020

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