Establishment of Regeneration Protocol for Canola (Brassica napus L.)

Establishment of Regeneration Protocol for Canola (Brassica napus L.)

Sabrina Shameen Alam

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Laila Khaleda

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Mohammad Al-Forkan

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University of Chittagong

Establishment of Regeneration Protocol for Canola (Brassica napus L.)

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Abstract

The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is not grown in Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4-dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl-1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).

References

10 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Sabrina Shameen Alam. 2013. \u201cEstablishment of Regeneration Protocol for Canola (Brassica napus L.)\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 13 (GJSFR Volume 13 Issue C7): .

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GJSFR Volume 13 Issue C7
Pg. 7- 12

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

and ms medium canola plant regeneration

Submission ReceivedOctober 19, 2013
Peer Review Double Blind
Handling Editor
Accepted November 13, 2013
Published November 24, 2013

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v1.2

Issue date

December 31, 2013

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Article Matrices

Total Score: 103

Country: Bangladesh

Subject: Global Journal of Science Frontier Research - C: Biological Science

Authors: Sabrina Shameen Alam, Laila Khaleda, Mohammad Al-Forkan (PhD/Dr. count: 0)

View Count (all-time): 138

Total Views (Real + Logic): 4693

Total Downloads (simulated): 2310

Publish Date: 2013 12, Tue

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Total Views: 4693

Total Downloads: 2310

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