Establishment of Regeneration Protocol for Canola (Brassica napus L.)

Establishment of Regeneration Protocol for Canola (Brassica napus L.)

Sabrina Shameen Alam

Contact

Laila Khaleda

Contact

Mohammad Al-Forkan

Contact

University of Chittagong

Establishment of Regeneration Protocol for Canola (Brassica napus L.)

Article Fingerprint

ReserarchID

B79HK

Establishment of Regeneration Protocol for Canola (Brassica napus L.) Banner

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

The study was conducted with an aim of developing a genotype independent efficient in vitro regeneration system for Canola (Brassica napus L.) that is not grown in Bangladesh. Plant regeneration was achieved through embryogenic callus production from hypocotyls and cotyledonary leaves with petioles. The optimum medium for callus induction was found with 0.5 mgl-1 2, 4-dichlorophenoxyacetic acid (2, 4-D). The best shooting medium for canola contained 3.0 mgl-1 benzyl amino purine (BAP), 0.1 mgl-1 naphtalene acetic acid (NAA), and 5.0 mgl-1 AgNO3 and produced maximum number of shoots when kinetin was used at a concentration of 0.5 mgl-1. The profound positive effect of AgNO3 for Canola was found for callogenesis at 0.5 mgl-1 and for regeneration at 5.0 mgl-1. In vitro regenerated shoots of canola produced well developed root system on both the media with 2.5 mgl-1 NAA and 1.0 mgl-1 indole 3-butyric acid (IBA).

References

10 Cites in Article

Reference Format

A Agnihotri,D Prem,K Gupta (2004). Biotechnology in Quality improvement of Brassicas.
H Ali,Z Ali,S Mehmood,W Ali (2007). In vitro regeneration of Brassica napus L. cultivars (Star, Cyclone and Westar) from hypocotyls and cotyledonary leaves.
Dilip Bhadra (2005). An Analysis on the Recent Terrorist Attacks in Bangladesh, Foreign Travelers’ Perceptions, Attitudes & Behaviour to Local Residents and Tourism Development & Sustainability'.
V Cardoza,C Stewart (2003). Increased Agrobacterium-mediated transformation & rooting efficiencies in canola (Brassica napus L.) from hypocotyl segment explants.
N Eskin,B Mcdonald (1991). Canola Oil.
J Ian,Adrian,D (2003). Comparison of genetically modified and conventionally derived herbicide tolerance in oilseed rape: A case study.
M Khan,H Rashid,M Ansar,Z Chaudry (2003). High frequency shoot regeneration and Agrobacterium-mediated DNA transfer in canola (Brassica napus).
Toshio Murashige,Folke Skoog (1962). A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue Cultures.
P Salisbery,N Wratten (1999). Canola In Australia; the first thirty years.
J Williams,D Pink,N Biddington (1990). Effect of silver nitrate on long-term culture and regeneration of callus from Brassica oleracea var. gemmifera.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Sabrina Shameen Alam. 2013. \u201cEstablishment of Regeneration Protocol for Canola (Brassica napus L.)\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 13 (GJSFR Volume 13 Issue C7): .

More Citation Formats

Select Citation Style:

Download Citation

Download Article

GJSFR Volume 13 Issue C7
Pg. 7- 12

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

and ms medium canola plant regeneration

Submission ReceivedOctober 19, 2013
Peer Review Double Blind
Handling Editor
Accepted November 13, 2013
Published November 24, 2013

Version of record

v1.2

Issue date

December 31, 2013

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 103

Country: Bangladesh

Subject: Global Journal of Science Frontier Research - C: Biological Science

Authors: Sabrina Shameen Alam, Laila Khaleda, Mohammad Al-Forkan (PhD/Dr. count: 0)

View Count (all-time): 140

Total Views (Real + Logic): 4695

Total Downloads (simulated): 2375

Publish Date: 2013 12, Tue

Monthly Totals (Real + Logic):

Month 1: 54 views
Month 2: 44 views
Month 3: 33 views
Month 4: 57 views
Month 5: 38 views
Month 6: 31 views
Month 7: 30 views
Month 8: 41 views
Month 9: 40 views
Month 10: 25 views
Month 11: 31 views
Month 12: 18 views
Month 13: 44 views
Month 14: 18 views
Month 15: 32 views
Month 16: 32 views
Month 17: 22 views
Month 18: 25 views
Month 19: 43 views
Month 20: 23 views
Month 21: 33 views
Month 22: 20 views
Month 23: 11 views
Month 24: 28 views
Month 25: 25 views
Month 26: 22 views
Month 27: 44 views
Month 28: 22 views
Month 29: 21 views
Month 30: 23 views
Month 31: 24 views
Month 32: 20 views
Month 33: 13 views
Month 34: 44 views
Month 35: 37 views
Month 36: 40 views
Month 37: 27 views
Month 38: 24 views
Month 39: 33 views
Month 40: 40 views
Month 41: 24 views
Month 42: 26 views
Month 43: 33 views
Month 44: 35 views
Month 45: 27 views
Month 46: 25 views
Month 47: 37 views
Month 48: 37 views
Month 49: 33 views
Month 50: 46 views
Month 51: 39 views
Month 52: 30 views
Month 53: 27 views
Month 54: 35 views
Month 55: 24 views
Month 56: 32 views
Month 57: 22 views
Month 58: 22 views
Month 59: 19 views
Month 60: 43 views
Month 61: 21 views
Month 62: 13 views
Month 63: 22 views
Month 64: 34 views
Month 65: 36 views
Month 66: 30 views
Month 67: 27 views
Month 68: 19 views
Month 69: 44 views
Month 70: 30 views
Month 71: 31 views
Month 72: 23 views
Month 73: 15 views
Month 74: 37 views
Month 75: 25 views
Month 76: 33 views
Month 77: 44 views
Month 78: 38 views
Month 79: 35 views
Month 80: 20 views
Month 81: 27 views
Month 82: 37 views
Month 83: 21 views
Month 84: 18 views
Month 85: 36 views
Month 86: 36 views
Month 87: 38 views
Month 88: 47 views
Month 89: 27 views
Month 90: 32 views
Month 91: 40 views
Month 92: 25 views
Month 93: 29 views
Month 94: 29 views
Month 95: 37 views
Month 96: 25 views
Month 97: 30 views
Month 98: 23 views
Month 99: 15 views
Month 100: 22 views
Month 101: 46 views
Month 102: 35 views
Month 103: 39 views
Month 104: 32 views
Month 105: 36 views
Month 106: 27 views
Month 107: 37 views
Month 108: 28 views
Month 109: 29 views
Month 110: 25 views
Month 111: 19 views
Month 112: 28 views
Month 113: 37 views
Month 114: 20 views
Month 115: 35 views
Month 116: 25 views
Month 117: 23 views
Month 118: 28 views
Month 119: 41 views
Month 120: 42 views
Month 121: 19 views
Month 122: 38 views
Month 123: 29 views
Month 124: 33 views
Month 125: 46 views
Month 126: 31 views
Month 127: 22 views
Month 128: 14 views
Month 129: 43 views
Month 130: 17 views
Month 131: 25 views
Month 132: 42 views
Month 133: 36 views
Month 134: 24 views
Month 135: 21 views
Month 136: 44 views
Month 137: 24 views
Month 138: 35 views
Month 139: 34 views
Month 140: 34 views
Month 141: 17 views
Month 142: 43 views
Month 143: 24 views
Month 144: 18 views
Month 145: 41 views
Month 146: 36 views
Month 147: 42 views
Month 148: 48 views
Month 149: 45 views

Total Views: 4695

Total Downloads: 2375

2026 Trends

Published Article

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]