Evaluation of the Antibacterial Activity in Pomegranate Peels and Arils by using Ethanolic Extract against S. mutans and L. acidophilus

1
Adith Venugopal
Adith Venugopal
2
Fatemeh Nikfallah
Fatemeh Nikfallah
3
Harsh Tejani
Harsh Tejani
4
Hemanth T. Lakshmikantha
Hemanth T. Lakshmikantha
1 University of the East, Manila, Philippines.

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The use of antibiotics has revolutionized the treatment of various enteric bacterial infections. However, their indiscriminate use has led to an alarming increase in antibiotic resistance among microorganisms, thus necessitating the need for development of novel antimicrobials. Then main objective of this study is to evaluate antibacterial activity of pomegranate fruit extract on selected bacterial culture. Antibacterial activity of pomegranate was tested on MRS agar plates by employing punch well technique. Various concentrations of the peels, arils and peels and arils mixture (1:1) prepared by dissolving in Dimethyl Sulphoxide to obtain a final concentration of 10g.ml, 5g.ml, 2.5g.ml and 1.25g.ml against the test organisms. The sensitivity of bacterial strains to aqueous and alcoholic extracts of the peels and arils of Punica granatum calculated by measuring the diameter of inhibition zone.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Adith Venugopal. 2014. \u201cEvaluation of the Antibacterial Activity in Pomegranate Peels and Arils by using Ethanolic Extract against S. mutans and L. acidophilus\u201d. Global Journal of Medical Research - J: Dentistry & Otolaryngology GJMR-J Volume 14 (GJMR Volume 14 Issue J2): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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June 3, 2014

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English

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The use of antibiotics has revolutionized the treatment of various enteric bacterial infections. However, their indiscriminate use has led to an alarming increase in antibiotic resistance among microorganisms, thus necessitating the need for development of novel antimicrobials. Then main objective of this study is to evaluate antibacterial activity of pomegranate fruit extract on selected bacterial culture. Antibacterial activity of pomegranate was tested on MRS agar plates by employing punch well technique. Various concentrations of the peels, arils and peels and arils mixture (1:1) prepared by dissolving in Dimethyl Sulphoxide to obtain a final concentration of 10g.ml, 5g.ml, 2.5g.ml and 1.25g.ml against the test organisms. The sensitivity of bacterial strains to aqueous and alcoholic extracts of the peels and arils of Punica granatum calculated by measuring the diameter of inhibition zone.

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Evaluation of the Antibacterial Activity in Pomegranate Peels and Arils by using Ethanolic Extract against S. mutans and L. acidophilus

Fatemeh Nikfallah
Fatemeh Nikfallah
Adith Venugopal
Adith Venugopal University of the East, Manila, Philippines.
Harsh Tejani
Harsh Tejani
Hemanth T. Lakshmikantha
Hemanth T. Lakshmikantha

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