## I. INTRODUCTION Tobacco leaves were processed in various methods in order to obtain high-quality products with pleasant taste sensations[1]. Usually various gases, vapors and liquid solutions are used for removing the bitterness of tobacco. Simultaneously, the use of chemical reagents does not introduce the desired changes in the composition of tobacco, and the complexity and duration of these procedures make them unprofitable. The closest to the technical solution is a method of processing tobacco, according to which it is cooled in a sealed container to a temperature of $T = 230$ K with the container immersed in a cooling mixture (solid $\mathrm{CO}_{2} +$ acetone) [2]. However, this method also does not allow to remove the bitterness and burning of tobacco, but rather removes nicotine and tar, the presence of which in a certain amount in tobacco is necessary. This goal is achieved by freezing tobacco leaves with liquid nitrogen [3]. The combined action of a microwave field and low temperature (to $T = 77$ K) on tobacco leaves makes it possible to remove bitterness and pungency. It is known that the natural fermentation of cured tobacco leaves is the most important process of forming the quality of tobacco raw materials. In the fermentation process, traditional heat treatment is used. However, the supply of heat and its penetration into the tobacco material takes a lot of time and requires a lot of energy. Therefore, the development of fundamentally new methods to accelerate the fermentation process of tobacco raw materials is very relevant. The used method which includes exposure to an electromagnetic microwave field is, in fact, the only accelerated method for curing tobacco material today. At the same time, it is important that tobacco intensively absorbing electromagnetic waves, heat up everywhere. In other words, it is heated from the inner layers to the outer [4]. This method is carried out by simultaneous air blowing [5], which causes an acceleration of moisture transfer. The disadvantage of the known method is that the tobacco leaves obtained after drying remain green. Naturally, residual chlorophylls negatively affect the quality of tobacco. The moisture of tobacco leaf is one of the main parameters affecting the fermentation process and, consequently, the quality of tobacco. Therefore, we set ourselves the task of finding more efficient ways of drying. At the same time, it is necessary to consider the fact that dry tobacco loses its elasticity during improper processing, as a result of which economic losses increase, and excessively moist raw materials easily splash. Therefore, obtaining dry tobacco leaves with a given moisture content and with the best taste qualities is of particular importance. Moreover, green and dried fermented samples of tobacco leaves of the "Samsun, Trapezoid" type grown in Azerbaijan were used for the study. The tablets were obtained from finely ground samples of tobacco leaves to record radiothermolumine scence (RTL) spectra. The samples were irradiated with gamma radiation using $^{60}\mathrm{Co}$ gamma-isotope source for 3 hours $(10.8\mathrm{kGy})$ at $T = 77K$. In the present work, the effect of simultaneous low-temperature and microwave treatment on the number of tobacco leaves is studied by IR spectroscopy. ## II. EXPERIMENTS As an object of study, aromatic and skeleton types of green tobacco leaves, (Samsun, Trapezoid) grown in Azerbaijan, were selected. Low-temperature and microwave processing of tobacco leaves was carried out on a laboratory unit "UHF-tobacco" [6]. At the same time, tobacco leaves are rapidly cooled in liquid nitrogen $(T = 77\mathrm{K})$, placed in one layer on a microwave oven conveyor and subjected to microwave field treatment with a frequency of $400\mathrm{MHz}$ at a radiation power of $50\mathrm{kW}$. Microwave heating was carried out with simultaneous air blowing which previously purified from carbon dioxide, and light treatment with a wavelength of $400 - 700\mathrm{nm}$. The choice of the spectral range $400 - 700\mathrm{nm}$ is due to the fact that the absorption band of residual chlorophyll is at $665\mathrm{nm}$ and photoirradiation of tobacco leaves in this region does not lead to a deterioration in the quality of tobacco [7]. IR spectra of tobacco pressed with KBr in the form of tablets were measured using Specord-71 IR spectrometer. ## III. RESULTS AND DISCUSSION It is known that the tobacco leaves are processed in various ways in order to obtain high-quality products with pleasant taste characteristics. Simultaneously, a number of investigations were carried out to remove the bitterness of tobacco under the influence of various gases, vapors and liquid solutions [7-8]. However, the use of chemical reagents leads to undesirable effects. Moreover, the complexity and duration of these procedures made them unprofitable.  Fig. 1: RTL curves for green (1) and dried fermented (2) tobacco leaves $(\mathrm{D} = 10\mathrm{kGy})$ The features of radiothermoluminescence (RTL) of green tobacco leaves in a wide temperature range of $80 \div 450\mathrm{K}$ have been studied. The luminescence curves of green tobacco leaves are characterized by the presence of maxima at temperatures of 170 and $350\mathrm{K}$ (Figure 1, curve 1). After curing and fermentation of tobacco leaves, the intensity of all peaks decreases, a broad and diffuse peak (half-width $\Delta T_{1/2} \sim 35\mathrm{K}$ ) at $\mathrm{T} = 350\mathrm{K}$ split into two comparative narrow peaks with maxima at $\mathrm{T} = 320\mathrm{K}$ ( $\Delta T_{1/2} \sim 35\mathrm{K}$ ) and $\mathrm{T} = 360\mathrm{K}$ ( $\Delta T_{1/2} \sim 25\mathrm{K}$ ) and new RTL peaks appear with a maximum at $\mathrm{T} = 220\mathrm{K}$ (Figure 1, curve 2). The appearance of a low-temperature peak at $\mathrm{T} = 170\mathrm{K}$ may be due to the presence of structural moisture in the tobacco composition. This is evidenced by a decrease in its intensity by $\sim 2.5$ times and a shift of the maximum by $15\mathrm{K}$ towards lower temperatures as the degree of curing increases. Apparently, molecular water, which is in the steric environment of alkaloids, has an indirect effect on the taste of tobacco. In the literature, another works were devoted to the technical solution of this survey. According to one of them, tobacco is cooled in the geometric container to a temperature of $230\mathrm{K}$ with the container immersed in the cooling mixture (solid $\mathrm{CO}_{2}$ + acetone). However, the disadvantage of the method is that tobacco loses a significant part of its nicotine and tar during such technical processing, which the presence of these components in a certain amount is extremely necessary.  Fig. 2: IR absorption spectra of tobacco leaves of the Trapezond type, original (1) and treated with a microwave field at a temperature of $T = 77K$ (2) It should be noted that the influence of the microwave field leads to a change in the chemical composition of tobacco leaves. This change is clearly seen in the IR spectra. Comparison of the IR spectra of the original and microwave-treated tobacco leaves shows that the main changes are observed in the region of $1000 - 200\mathrm{cm}^{-1}$ (Fig. 2). According to (5, 6), the 1390 $\mathrm{cm}^{-1}$ band refers to bending vibrations of $\mathrm{CH}_3$ groups, and $1460~\mathrm{cm}^{-1}$ to bending vibrations of common alkyl $(\mathrm{CH}_3$ and $\mathrm{CH}_2)$ groups that make up lipid-saturated fatty acids, described by the formula R-COOH with linear and non-linear carbon chains $\mathsf{R} = \mathsf{CH}_{3},(\mathsf{CH}_{2})\mathsf{n}$ In low-grade tobacco, carbon chain branching is mainly the accumulation of lower acids, which adversely affect the quality of tobacco. When treated with a microwave field, the total intensities of the 1390 and $1460~\mathrm{cm}^{-1}$ bands are redistributed and reduced, which is associated with the breaking of branched chains and a decrease in their lengths. From the change in the intensities of the bands at 1250 and $1320~\mathrm{cm}^{-1}$, which is a consequence of the superposition of in-plane deformation vibrations of CO and OH groups, one can judge the proximity of the chain branching site to the carboxyl group. These changes are indicative of the degree of improvement in the quality of processed tobacco leaves. In the original tobacco leaves, a significant amount of protein causes a strong taste of bitterness, which negatively affects the quality. The main protein macromolecules are amino acids, which have two types of functional groups - acidic carboxyl - C-O-OH - basic amino group $(-NH_{2}, - NH)$ In the IR spectra, the stretching vibrations of the amino groups are at $3300~\mathrm{cm}^{-1}$, and the bending vibrations are in the frequency range of 1590-1650 cm $^{-1}$ (Fig. 1). We have tried to remove bitterness and pungency by the combined action of a microwave electromagnetic field and low temperature (77 K). Simultaneously, tobacco leaves cooled in liquid nitrogen were treated with a microwave field and the change in the chemical composition of tobacco leaves was traced by the IR spectroscopy of the sample. By changing the intensities of the bands associated with the breaking of branched chains, the amount of lower acids that adversely affect the quality of tobacco was determined. It should be noted that the low-temperature microwave treatment proposed by us did not lead to a significant change in the structure of various carbohydrates containing catonic and functional aldehyde groups. In addition, it is known that the quality of the finished tobacco product is strongly influenced by the curing and fermentation processes. At the same time, residual chlorophyll significantly reduces its quality. Therefore, we considered it expedient to improve the quality of the finished product by accelerating the decomposition of chlorophyll. This goal was achieved by us by searching for new methods of curing and fermentation, devoid of shortcomings that adversely affect the quality of the product. The searches have shown that one of these methods is the dielectric heating of tobacco, since mainly dielectric heating is strongly manifested on materials with low thermal conductivity and heat-resistant properties. It is clear that the impact on tobacco leaves - as a dielectric of electromagnetic fields would allow the flow of photo - and thermochemical reactions not only on the surface, but throughout the entire volume. The intensity of deformation vibrations of amino groups decreases by $\div 1.5$ times after low-temperature microwave treatment of tobacco leaves. This is due to a decrease in the number of proteins of various structures. However, low-temperature microwave treatment does not lead to a significant change in the structure of various carbohydrates containing catonic and aldehyde functional groups (-C-O-H, -C-O). Therefore, the intensity of the band at $1750~\mathrm{cm}^{-1}$ practically does not change. Comparison of the relative changes in the intensities of the carbohydrate and protein bands of the original and processed tobacco leaves shows that the values of the percentage of carbohydrates to proteins increase. This once again indicates an improvement in the quality of tobacco after low-temperature microwave processing. It should be noted that one of the advantages of microwave curing is that, in addition to local thermal effects, the electromagnetic field also induces the probability of uniform photochemical reactions of individual enzymes, which makes it possible to obtain high-quality products. It is known that the chemical composition of tobacco leaves changes during fermentation. The color is gradually leveling out, approaches monophonic and the green disappears. Simultaneously, a high residual content of chlorophyll leads to the accumulation of substances that adversely affect the quality of tobacco. Based on the above material, we are concluded that it is advisable to accelerate the decomposition of residual chlorophyllin order to improve the quality of the finished product. We achieved this goal by the fact that microwave heating, driven at a frequency of 400-1000 MHz, was carried out with simultaneous blowing with air which previously purified from carbon dioxide and treatment with light with a wavelength of 400-700 nm. The proposed method of curing tobacco allowed us to obtain dry tobacco leaves with yellowish color, which met the requirements for high-grade tobacco products. This was achieved due to the fact that tobacco leaves were irradiated with light with a wavelength that causes photooxidation of green pigments. At the same time, simultaneous blowing with air purified from carbon dioxide sharply suppressed the natural process of photosynthesis in tobacco leaves, and the frequency of the used electromagnetic field provided the necessary duration of processing and curing of the leaves. Our experiments have shown that curing tobacco at a microwave field frequency below $400\mathrm{MHz}$ is undesirable, since this slows down the process of removing moisture from the leaves. Curing at a frequency above $1000\mathrm{MHz}$ is also undesirable, since the rate of moisture removal from the leaves exceeds the rate of photooxidation of green leaves, due to which the finished product retains its green color. Since combustibility is one of the most important qualitative characteristics of tobacco raw materials, we investigated the conditions for this process to occur in tobacco processed using above described method. A comprehensive study of the tobacco combustion process was carried out by X-ray diffraction methods, infrared-photoelectron spectroscopy, as well as differential thermal analysis. Based on the determination of temperature intervals for exothermic effects, criteria were established both for the tobacco fermentation and for its degree. Studying the processes of pyrolysis and oxidation of tobacco, as well as determining the temperature transitions of the decomposition of its components with an analysis of the composition and structure, after each stage of pyrolysis, makes it possible to purposefully control the content of alkaloids (in particular, nicotine) and tar in tobacco, as well as to conduct comparative assessments of their quality. The obtained data on the combustibility of tobacco also correlate well with the data on the microelement composition of tobacco preparations obtained by us using spectral analysis method. Atomic adsorption method has shown that the studied tobacco samples contain trace elements, such as - Pb, Sn, Cr, Mn, Ni, Al, Ti, Si, Mg, Ca, Mo, K, Cu, Zr, Na, Co, Sr, Fe, V, Y. It was found that Si (15-17%), Al (-2%), Mg (-3%), Ca (-9%) and Na (more than 1%). A certain correlation was found between the amount of K and Fe and the tobacco variety. It is shown that the highest grade of tobacco is characterized by a higher content of K and a relatively low content of Fe. The investigated samples of tobacco had practically no chlorine and sulfur-containing compounds. It is concluded that the types of tobacco grown in the territory of Azerbaijan have a rich microelement composition and they are characterized by higher quality indicators [8].

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