Fluorescence, in microscopy and imaging

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Dikedi P.N.
Dikedi P.N. B.Sc., M.Sc., MRes, MINstp, FGS
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Dr. P. N. Dikedi
Dr. P. N. Dikedi
α King's College London King's College London

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Fluorescence, in microscopy and imaging

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Abstract

This is a swift report on fluorescence and its application as a tool in imaging and microscopy; to properly comprehend this subject, with the aid of the Jablonski diagram, brief descriptions and discussions are given on fluorescence; rhodamine 6G homo-FRET reaction is considered from which both the absorption and emission spectra are drawn on a graph. Additionally, the wealth of applications of fluorescence imaging techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Polarisation and Anisotropy techniques are considered. Applications of molecular imaging techniques in cell biology and medical science are mentioned.

References

8 Cites in Article
  1. J Lovell,T Liu,J Chen,G Zheng (2010). Activatable Photosensitizers for Imaging and Therapy.
  2. Klaus Suhling,Paul French,David Phillips (2004). Time-resolved fluorescence microscopy.
  3. Y Berezin,S Achilefu (2010). Fluorescence Lifetime Measurements and Biological Imaging.
  4. Samuel Achilefu (2010). Introduction to Concepts and Strategies for Molecular Imaging.
  5. J Han,Kevin Burgess*han,K (2010). Fluorescent Indicators for Intracellular pH.
  6. D Jameson,J Ross (2010). Fluorescence Polarization/Anisotropy in Diagnostics and Imaging.
  7. T Oida,Y Sako,A Kusumi (1993). Fluorescence lifetime imaging microscopy (flimscopy). Methodology development and application to studies of endosome fusion in single cells.
  8. Yinghua Sun,Jennifer Phipps,Daniel Elson,Heather Stoy,Steven Tinling,Jeremy Meier,Brian Poirier,Frank Chuang,D Farwell,Laura Marcu (2009). Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dikedi P.N.. 2012. \u201cFluorescence, in microscopy and imaging\u201d. Global Journal of Science Frontier Research - A: Physics & Space Science GJSFR-A Volume 12 (GJSFR Volume 12 Issue A3): .

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Issue Cover
GJSFR Volume 12 Issue A3
Pg. 27- 31
Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Version of record

v1.2

Issue date

April 7, 2012

Language
en
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This is a swift report on fluorescence and its application as a tool in imaging and microscopy; to properly comprehend this subject, with the aid of the Jablonski diagram, brief descriptions and discussions are given on fluorescence; rhodamine 6G homo-FRET reaction is considered from which both the absorption and emission spectra are drawn on a graph. Additionally, the wealth of applications of fluorescence imaging techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Polarisation and Anisotropy techniques are considered. Applications of molecular imaging techniques in cell biology and medical science are mentioned.

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Fluorescence, in microscopy and imaging

Dr. P. N. Dikedi
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