Fluorescence, in microscopy and imaging

Dr. P. N. Dikedi

Fluorescence, in microscopy and imaging

Dr. P. N. Dikedi * § B.Sc., M.Sc., MRes, MINstp, FGS

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King's College London

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Abstract

This is a swift report on fluorescence and its application as a tool in imaging and microscopy; to properly comprehend this subject, with the aid of the Jablonski diagram, brief descriptions and discussions are given on fluorescence; rhodamine 6G homo-FRET reaction is considered from which both the absorption and emission spectra are drawn on a graph. Additionally, the wealth of applications of fluorescence imaging techniques such as Fluorescence Lifetime Imaging Microscopy (FLIM) and Fluorescence Polarisation and Anisotropy techniques are considered. Applications of molecular imaging techniques in cell biology and medical science are mentioned.

References

8 Cites in Article

Reference Format

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Y Berezin,S Achilefu (2010). Fluorescence Lifetime Measurements and Biological Imaging.
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Yinghua Sun,Jennifer Phipps,Daniel Elson,Heather Stoy,Steven Tinling,Jeremy Meier,Brian Poirier,Frank Chuang,D Farwell,Laura Marcu (2009). Fluorescence lifetime imaging microscopy: in vivo application to diagnosis of oral carcinoma.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dr. Dikedi. 2012. "Fluorescence, in microscopy and imaging". Global Journal of Science Frontier Research, Global Journal of Science Frontier Research - A: Physics & Space Science GJSFR-A Volume 12 (GJSFR Volume 12 Issue A3).

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GJSFR Volume 12 Issue A3

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

Keywords

Anisotropy Fluorescence Polarisation rhodamine

Classification

GJSFR-A Classification FOR Code: 020402

Submission ReceivedJanuary 22, 2012
Peer Review Double Blind
Handling Editor
Accepted February 16, 2012
Published March 1, 2012

Version of record

v1.2

Issue date

April 7, 2012

Language

English

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Article Matrices

Total Score: 150

Country: Nigeria

Subject: Global Journal of Science Frontier Research

Authors: Dr. P. N. Dikedi (PhD/Dr. count: 1)

View Count (all-time): 292

Total Views (Real + Logic): 2156

Total Downloads (simulated): 143

Publish Date: 2012 03, Thu

Monthly Totals (Real + Logic):

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Total Views: 2.2K

Total Downloads: 143

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