FLUOXETINE BIOEQUIVALENCE STUDY: QUANTIFICATION OF FLUOXETINE BY LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY

1
dr.luciana_fernandes_duarte
dr.luciana_fernandes_duarte
2
Dr.Luciana Fernandes Duarte
Dr.Luciana Fernandes Duarte
3
Lara Cristina Silva
Lara Cristina Silva
4
Moiss L. P. Vanunci
Moiss L. P. Vanunci
5
Tatiane A. M. Stein e Marina M. Nakamura
Tatiane A. M. Stein e Marina M. Nakamura
1 State University of Campinas

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A liquid chromatography method was developed and validated for the determination of fluoxetine in human plasma using paroxetine as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C 18 analytical column, with water:acetonitrile as mobile phase, run at a flow rate of 0.20 mL/min. The method was linear in the range of 0.2-50 ng/mL and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations of fluoxetine 20 mg after single oral dose administration to healthy human volunteers.

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dr.luciana_fernandes_duarte. 1970. \u201cFLUOXETINE BIOEQUIVALENCE STUDY: QUANTIFICATION OF FLUOXETINE BY LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY\u201d. Unknown Journal GJMR Volume 10 (GJMR Volume 10 Issue 2): .

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August 10, 2010

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A liquid chromatography method was developed and validated for the determination of fluoxetine in human plasma using paroxetine as internal standard. The drugs were extracted from plasma by liquid-liquid extraction and separated isocratically on a C 18 analytical column, with water:acetonitrile as mobile phase, run at a flow rate of 0.20 mL/min. The method was linear in the range of 0.2-50 ng/mL and demonstrated acceptable results for the precision, accuracy and stability studies. The method was successfully applied for the bioequivalence study of two tablet formulations of fluoxetine 20 mg after single oral dose administration to healthy human volunteers.

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FLUOXETINE BIOEQUIVALENCE STUDY: QUANTIFICATION OF FLUOXETINE BY LIQUID CHROMATOGRAPHY COUPLED TO MASS SPECTROMETRY

Dr.Luciana Fernandes Duarte
Dr.Luciana Fernandes Duarte
Lara Cristina Silva
Lara Cristina Silva
Moiss L. P. Vanunci
Moiss L. P. Vanunci
Tatiane A. M. Stein e Marina M. Nakamura
Tatiane A. M. Stein e Marina M. Nakamura

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