Functional Dietary Supplementation of Okara soybeans residue on Streptozotocin Induced Diabetes Mellitus in Male Wistar Rats
A poor dietary habit has been demonstrated to be one of the key players in the development of diabetes mellitus, and a diet rich in dietary fiber has been highlighted to be a potent candidate for the management of diabetes mellitus. Therefore, this study is aimed to validate the role of dietary supplementation of okara (soybeans residue) in streptozotocin induced diabetic male Wistar rats. The total of 28 rats between the weight of 100 to 105g, was grouped into four n=7, and this study spanned for a period of 43days. All experimentations were conducted using standard method, and our findings show that the cumulative feed intake of 15% okara diet supplementation was significantly higher p<0.05 particularly from day 29 to day 43 relative to the negative control. After treatment for a period of 43days, 6mg/kg glibenclamide treated group 226.33±6.38 and 15% okara supplemented diet fed group 219.83±5.67 showed a significant increase p<0.05 in body weight relative to the Negative control 161.17±3.60. 15% okara diet supplementation significantly lowered p<0.05 blood sugar levels after treatment relative to after induction similar to 6mg/kg glibenclamide treated group. Glycated hemoglobin, glucose-6-phosphate dehydrogenase, lipid profile (CHOL, TRIG, LDL and HDL), liver function enzymes (AST, ALT, ALP and GGT), kidney function biomarkers (Creatinine, Urea, Sodium and potassium) and antioxidant enzymes (CAT, SOD, GSH, GST and GPX) were all significantly restored within the normal range. Histological observations of the pancreatic, liver, and kidney tissue showed no visible lesion for 15% Okara supplemented diet feeding. Conclusively, we recommend food supplementation with Okara.
