Growth Curves of Filamentous Fungi for Utilization in Biocatalytic Reduction of Cyclohexanones

1
lenilson Coutinho
lenilson Coutinho
2
Gliseida ZelayarAn Melgar
Gliseida ZelayarAn Melgar
3
Francisca Vanessa Souza De Assis
Francisca Vanessa Souza De Assis
4
Lenilson Coutinho Da Rocha
Lenilson Coutinho Da Rocha
5
Silmara Cristina Fanti
Silmara Cristina Fanti
6
Lara DurAes Sette
Lara DurAes Sette
7
Andr Luiz Melei
Andr Luiz Melei
1 University of SAo Paulo

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The growth of filamentous fungal mass in malt extract culture was recorded over 10 days at 32 o C, with the aim of utilizing the mycelium for the biocatalytic reduction of cyclohexanones1-3. Growth curves were obtained for marine fungi (Aspergillus sydowii CBMAI 933, Penicillium miczynskii CBMAI 930 and Trichoderma sp. CBMAI 932) and terrestrial fungi (Rhizopus sp. Cs1 and Aspergillus sp. Pd1), plotted asusing mycelialdry weight. The mycelia of Aspergillus sp. and Rhizopus sp. catalyzed the biocatalytic reduction of cyclohexanones1-3efficiently, when these substrates were added inthe exponential phase of growth at(72 h). The bioreduction of 4-methylcyclohexanone (3) by Aspergillus sp. and Rhizopus sp. yielded preferentially the cis-4-methylcyclohexanol (6), a thermodynamically and kinetically less stable compound. Whole cells of Aspergillus sp. and Rhizopus sp. showed good enzymatic activities in the bioreduction of ketone 1, yielding trans-diol 4.

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The authors declare no conflict of interest.

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lenilson Coutinho. 2013. \u201cGrowth Curves of Filamentous Fungi for Utilization in Biocatalytic Reduction of Cyclohexanones\u201d. Global Journal of Science Frontier Research - B: Chemistry GJSFR-B Volume 13 (GJSFR Volume 13 Issue B5): .

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GJSFR Volume 13 Issue B5
Pg. 13- 19
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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August 3, 2013

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English

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The growth of filamentous fungal mass in malt extract culture was recorded over 10 days at 32 o C, with the aim of utilizing the mycelium for the biocatalytic reduction of cyclohexanones1-3. Growth curves were obtained for marine fungi (Aspergillus sydowii CBMAI 933, Penicillium miczynskii CBMAI 930 and Trichoderma sp. CBMAI 932) and terrestrial fungi (Rhizopus sp. Cs1 and Aspergillus sp. Pd1), plotted asusing mycelialdry weight. The mycelia of Aspergillus sp. and Rhizopus sp. catalyzed the biocatalytic reduction of cyclohexanones1-3efficiently, when these substrates were added inthe exponential phase of growth at(72 h). The bioreduction of 4-methylcyclohexanone (3) by Aspergillus sp. and Rhizopus sp. yielded preferentially the cis-4-methylcyclohexanol (6), a thermodynamically and kinetically less stable compound. Whole cells of Aspergillus sp. and Rhizopus sp. showed good enzymatic activities in the bioreduction of ketone 1, yielding trans-diol 4.

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Growth Curves of Filamentous Fungi for Utilization in Biocatalytic Reduction of Cyclohexanones

Gliseida ZelayarAn Melgar
Gliseida ZelayarAn Melgar
Francisca Vanessa Souza De Assis
Francisca Vanessa Souza De Assis
Lenilson Coutinho Da Rocha
Lenilson Coutinho Da Rocha
Silmara Cristina Fanti
Silmara Cristina Fanti
Lara DurAes Sette
Lara DurAes Sette
Andr Luiz Melei
Andr Luiz Melei

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