In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

Somayeh Afsah Vakili

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Ajay George

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Syed Fayazuddin

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α Rajiv Gandhi University of Health Sciences

In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

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Abstract

In spite of the fact that substantial advancement have been made in the remedy and control of cancer progression, remarkable inadequacy for improvement remain. Natural therapy can diminish adverse effect of chemotherapy. Currently over 60% of the drugs are derived in one or other way from natural source including plant, marine organism and micro-organism. The present investigation was concerned with pharmacological potential of honey bee venom and ethanol extract of Zingiber officinale Roscoe towards anticancer activity. The cytotoxic potency of combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe was evaluated on cultured cancer cells Hep-2 by sulphoradamine B assay (IC 50 =53 μg/ml) and moreover, it was invested by brine shrimp lethality assay (IC 50 =48.31 μg/ml), tryphan blue exclusion assay (IC 50 =37.49 μg/ml), and MTT assay. MTT assay exhibited that combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe has more cytotoxicity potency towards human breast adenocarcinoma cells (MCF-7) than normal Chinese hamster lung fibroblast cells (V79).

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Somayeh Afsah Vakili. 2017. \u201cIn vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice\u201d. Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 17 (GJMR Volume 17 Issue B2): .

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GJMR Volume 17 Issue B2
Pg. 9- 18

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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Classification

GJMR-B Classification: NLMC Code: QS 679

Submission ReceivedDecember 7, 2016
Peer Review Double Blind
Handling Editor
Accepted January 4, 2017
Published January 15, 2017

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v1.2

Issue date

August 12, 2017

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Article Matrices

Total Score: 103

Country: India

Subject: Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine

Authors: Somayeh Afsah Vakili, Ajay George, Syed Fayazuddin (PhD/Dr. count: 0)

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Publish Date: 2017 08, Sat

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Total Views: 3383

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