In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

Somayeh Afsah Vakili

In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

Somayeh Afsah Vakili * §

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§ Visveswarapura Institute of Pharmaceutical Sciences,

In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice

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Abstract

In spite of the fact that substantial advancement have been made in the remedy and control of cancer progression, remarkable inadequacy for improvement remain. Natural therapy can diminish adverse effect of chemotherapy. Currently over 60% of the drugs are derived in one or other way from natural source including plant, marine organism and micro-organism. The present investigation was concerned with pharmacological potential of honey bee venom and ethanol extract of Zingiber officinale Roscoe towards anticancer activity. The cytotoxic potency of combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe was evaluated on cultured cancer cells Hep-2 by sulphoradamine B assay (IC 50 =53 μg/ml) and moreover, it was invested by brine shrimp lethality assay (IC 50 =48.31 μg/ml), tryphan blue exclusion assay (IC 50 =37.49 μg/ml), and MTT assay. MTT assay exhibited that combination of honey bee venom and ethanol extract of Zingiber officinale Roscoe has more cytotoxicity potency towards human breast adenocarcinoma cells (MCF-7) than normal Chinese hamster lung fibroblast cells (V79).

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Somayeh Vakili. 2017. "In vitro Cytotoxic Screening of Combination of Honey Bee Venom and Ethanol Extract of Zingiberaceae and i vivo Anticancer Evaluation of them against DLA and EAC Bearing Mice". Global Journal of Medical Research, Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 17 (GJMR Volume 17 Issue B2).

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GJMR Volume 17 Issue B2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-B Classification NLMC Code: QS 679

Submission ReceivedDecember 7, 2016
Peer Review Double Blind
Handling Editor
Accepted January 4, 2017
Published January 15, 2017

Version of record

v1.2

Issue date

August 12, 2017

Language

English

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Article Matrices

Total Score: 143

Country: India

Subject: Global Journal of Medical Research

Authors: Somayeh Afsah Vakili, Ajay George, Syed Fayazuddin (PhD/Dr. count: 0)

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