In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

Ajibade

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V. A.

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Ajenifuja

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O. A.

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In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

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Abstract

The antibacterial potency of alkaloid extracted from Phyllanthus niruri was examined on Fluoroquinolone resistant Escherichia coli isolated from different clinical samples using disk diffusion method. Different concentrations (0.1 -5mg/ml) of the alkaloid were used. It was observed that at 0.5mg/ml the extract showed more potency on Escherichia coli isolated from urine than from other samples with a diameter of zone of inhibition of 25.5mm. The percentage susceptibility of the isolated bacterium from urine, blood, semen, swab, and high vagina swab (HVS) to the alkaloid were 75%, 75%, 100%, 60% and 60% respectively. Thirty-seven (37) strains were tested for extended-spectrum beta-lactamase (ESBL) identification. They were all positive for blaCTX-M in 37(100%) of the ESBL-carrying strains. CXT-M-14 was the most frequently isolated ESBL (n=15), followed by CTX-M-27 (n=12) and CTX-M-15(n=5), one strain (CEC7) was carrying both blaCTX-M-14and blaCTX-M-15. Strain CEC14 was carrying a blaCTX-M-14 variant, which differed from the parental enzyme by a single transversion. Using PCR amplification,4 clusters containing 9, 8, 3, and 2 strains were identified. Pulsed-field gel electrophoresis of FQ-resistant E.coli identified clonal spread of 1(one) strain among 18 patients. It was concluded that all the bacterium resistant to fluoroquinolone were susceptible to the alkaloid extract.

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Reference Format

Corinne Arpin,Laure Coulange,Véronique Dubois,Catherine André,Isabelle Fischer,Sophie Fourmaux,Frédéric Grobost,Jacqueline Jullin,Brigitte Dutilh,Jean-Francois Couture,Patrick Noury,Isabelle Lagrange,Aline Ducastaing,Henri-Pierre Doermann,Claudine Quentin (2007). Extended-Spectrum-β-Lactamase-Producing <i>Enterobacteriaceae</i> Strains in Various Types of Private Health Care Centers.
M Barros,N Schor,M Boim (2003). Effects of an aqueous extract from Phyllantus niruri on calcium oxalate crystallization in vitro.
E Calbo,V Romani,M Xercavins,L Gomez,C Vidal,S Quintana,J Vila (2006). Risk factors for community-onset urinary tract infections due to Escherichia coli harbouring extended-spectrum 4.
M Dhir,L; Crayg,F Berman (2000). Screening of Indian Plants for biological activity.
R; Etienne,Sopheak,L; H; Sovannarith,G; Valerie,A; Frederic,S; Jean-Louis,M Didier,Guillaume (2009). CTX-M β-Lactamase in Escherichia coli from community-acquired Urinary tract infections.
D George,M Pamplona-Roger (2002). Encyclopaedia of Medicinal Plants.
B Halim,A Ali (2002). Oral hypoglycaemic effect of Phyllanthus niruri Linn.
Johnson Menard,M; Johnson,B; Kuskowaski,M A; Nichol,K; Zhanel,G (2009). Epidermic Clonal groups of Escherichia coli as a cause of antimicrobial-resistant urinary tract infections in Canada, 2002-2004.
A Khanna,F Rizvi,R Chander (2002). Lipid lowering activity of Phyllanthus niruri in hyperlipemic rats.
E Lautenbach,J Patel,W Bilker,P Edelstcin,N Fishman (2001). Extended-spectrum βlactamase producing Escherichia coli and Klebsiella pneumoniae: risk factors for infection and impact of resistance on out comer.
E Lauterbach,N Fishman,W Bilker,A Castiglioni,J Metlay,P Edelstein (2002). Risk factors for fluoroquinolone resistance in nosocomial Escherichia coli and KlebsiellaClin infect.
J Maslow,E Lautenbach,T Glaze,W Bilker,J Johnson (2004). Colonization of extraintestinal pathogenic Escherichia coli among nursing home residents and its relationship to FQresistance.
A Naik,A Juvekar (2003). Effects of alkaloidextract of Philanthusnirurion HIV replication.
H Odetola,O Okorosobo (1996). Preliminary in vitro antimicrobial activity of two Nigeria Diospyro species.
P Qudhia,R Tripathi (2002). Prospect for cultivation of medicinal plants in Chattisgarh, Indian.
S Tharakan,P Binitha,R Kuttan,G Kuttan (2000). ANTIVIRAL ACTIVITY OF HERBAL IMMUNOMODULATORY PREPARATIONS NCV I AND AC II – AND THEIR USEFULNESS IN HIV INFECTION.
N Tabasum,S Chatterrvedi,S Aggrawal,N Ahmed (2005). Hepatoprotective studies of Phyllanthusnirurion paracetamol-induced liver cell damage in albino mice.
F Tenover,R Arbeit,R Goering,P Mickelsen,B Murray,D Persing,B Swaminathan (1995). Interpreting chromosomal DNA restriction patterns produced by pulsed-field gel electrophoresis: criteria for bacterial strain typing.
A Tiwalade,O Abiodun,B Nicolas,P Mary,Mari-Therese,M (2000). Petersaponins III and IV Triterpenoidsaponins from Petersianthusmacrocarpus.
David Unander,P Venkateswaran,Irving Millman,Herbert Bryan,Baruch Blumberg (1990). <i>Phyllantus</i> Species: Sources of New Antiviral Compounds.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

. 2013. \u201cIn-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 13 (GJMR Volume 13 Issue C3): .

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GJMR Volume 13 Issue C3
Pg. 13- 16

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

alkaloid escherichia coli fluoroquinolone In-vitro Phyllanthus niruri

Submission ReceivedDecember 13, 2012
Peer Review Double Blind
Handling Editor
Accepted January 4, 2013
Published January 15, 2013

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August 10, 2013

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Total Score: 104

Country: Unknown

Subject: Global Journal of Medical Research - C: Microbiology & Pathology

Authors: Ajibade, V. A., Ajenifuja, O. A. (PhD/Dr. count: 0)

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