In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

Ajibade

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V. A.

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Ajenifuja

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O. A.

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In-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri

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Abstract

The antibacterial potency of alkaloid extracted from Phyllanthus niruri was examined on Fluoroquinolone resistant Escherichia coli isolated from different clinical samples using disk diffusion method. Different concentrations (0.1 -5mg/ml) of the alkaloid were used. It was observed that at 0.5mg/ml the extract showed more potency on Escherichia coli isolated from urine than from other samples with a diameter of zone of inhibition of 25.5mm. The percentage susceptibility of the isolated bacterium from urine, blood, semen, swab, and high vagina swab (HVS) to the alkaloid were 75%, 75%, 100%, 60% and 60% respectively. Thirty-seven (37) strains were tested for extended-spectrum beta-lactamase (ESBL) identification. They were all positive for blaCTX-M in 37(100%) of the ESBL-carrying strains. CXT-M-14 was the most frequently isolated ESBL (n=15), followed by CTX-M-27 (n=12) and CTX-M-15(n=5), one strain (CEC7) was carrying both blaCTX-M-14and blaCTX-M-15. Strain CEC14 was carrying a blaCTX-M-14 variant, which differed from the parental enzyme by a single transversion. Using PCR amplification,4 clusters containing 9, 8, 3, and 2 strains were identified. Pulsed-field gel electrophoresis of FQ-resistant E.coli identified clonal spread of 1(one) strain among 18 patients. It was concluded that all the bacterium resistant to fluoroquinolone were susceptible to the alkaloid extract.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

. 2013. \u201cIn-Vitro Susceptibility of Fluoroquinolone Resistance Escherichia Coli to Alkaloid Extracted from Phyllanthusniruri\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 13 (GJMR Volume 13 Issue C3): .

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GJMR Volume 13 Issue C3
Pg. 13- 16

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

alkaloid escherichia coli fluoroquinolone In-vitro Phyllanthus niruri

Submission ReceivedDecember 13, 2012
Peer Review Double Blind
Handling Editor
Accepted January 4, 2013
Published January 15, 2013

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August 10, 2013

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Total Score: 104

Country: Unknown

Subject: Global Journal of Medical Research - C: Microbiology & Pathology

Authors: Ajibade, V. A., Ajenifuja, O. A. (PhD/Dr. count: 0)

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