Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

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E4CZU

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Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

Amy Sun Merck & Co., Inc.

Raymond L. Lam

Amy Harman

Anna C. Pavlick

Gary A. Herman

Lisa M. Dauffenbach

Christopher A. Kerfoot

Pearl Huang

Jonathan Cheng

Tim Demuth

Robert Iannone

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Abstract

This study was to clinically validate phosphorylated CDC2(pCDC2) as a biomarker for Wee1 kinase inhibitors by measuring pCDC2 in skin biopsies from patients receiving DNA damaging chemotherapy. Skin biopsies were performed at scheduled times after chemotherapy. Total CDC2 and pCDC2 in epidermal cells, hair follicle and bulb from skin biopsies were determined using chromogenic multiplex immunohis-tochemistry with multispectral image analysis. Statistical analyses were performed for each cell type after logtransformation of data. Thirty-one patients (29-88 years) completed the study. Significant induction of pCDC2 in response to chemotherapy was detected. Epidermis was most consistently evaluable across skin biopsies, demonstrating strong induction of pCDC2. The percentage of cells positive for total CDC2 and pCDC2 showed a 1.40-fold induction from baseline to 24h post-infusion (p=0.012) and a 2.05-fold increase from baseline to 48h (p

Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

Authors

Amy Sun Merck & Co., Inc.

Raymond L. Lam

Amy Harman

Anna C. Pavlick

Gary A. Herman

Lisa M. Dauffenbach

Christopher A. Kerfoot

Pearl Huang

Jonathan Cheng

Tim Demuth

Robert Iannone

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How to Cite

Amy Sun. 2014. “. Global Journal of Medical Research – K: Interdisciplinary GJMR-K Volume 14 (GJMR Volume 14 Issue K1): .

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

GJMR Volume 14 Issue K1
Pg. 11- 21

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Submission Received December 10, 2013
Accepted January 4, 2014
Published January 15, 2014

Keywords

biological markers biomarkers cancers Cell cycle proteins Cells chemotherapy phosphorylated CDC2 proteins

Article Matrices

Total Score: 141

Country: United States

Subject: Global Journal of Medical Research - K: Interdisciplinary

Authors: Amy Sun, Raymond L. Lam, Amy Harman, Anna C. Pavlick, Gary A. Herman, Lisa M. Dauffenbach, Christopher A. Kerfoot, Pearl Huang, Jonathan Cheng, Tim Demuth, Robert Iannone (PhD/Dr. count: 0)

View Count (all-time): 98

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Publish Date: 2014 03, Thu

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Total Views: 4749

Total Downloads: 2321

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