Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

Amy Sun; Raymond L. Lam; Amy Harman; Anna C. Pavlick; Gary A. Herman

Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

Amy Sun * §

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Raymond L. Lam §

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Amy Harman §

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Anna C. Pavlick §

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Gary A. Herman §

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§ Merck & Co., Inc.

Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy

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Abstract

This study was to clinically validate phosphorylated CDC2(pCDC2) as a biomarker for Wee1 kinase inhibitors by measuring pCDC2 in skin biopsies from patients receiving DNA damaging chemotherapy. Skin biopsies were performed at scheduled times after chemotherapy. Total CDC2 and pCDC2 in epidermal cells, hair follicle and bulb from skin biopsies were determined using chromogenic multiplex immunohis-tochemistry with multispectral image analysis. Statistical analyses were performed for each cell type after log-transformation of data. Thirty-one patients (29-88 years) completed the study. Significant induction of pCDC2 in response to chemotherapy was detected. Epidermis was most consistently evaluable across skin biopsies, demonstrating strong induction of pCDC2. The percentage of cells positive for total CDC2 and pCDC2 showed a 1.40-fold induction from baseline to 24h post-infusion (p=0.012) and a 2.05-fold increase from baseline to 48h (p

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Amy Sun, Raymond L. Lam, Amy Harman, Anna C. Pavlick, Gary A. Herman. 2014. "Induction of CDC2 Phosphorylation in Skin Biopsies from Patients with Solid Tumors Undergoing DNA-Damaging Chemotherapy". Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 14 (GJMR Volume 14 Issue 2 (K1)).

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GJMR Volume 14 Issue 2 (K1)

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

biological markers biomarkers cancers Cell cycle proteins Cells chemotherapy phosphorylated CDC2 proteins

Classification

GJMR-K Classification NLMC Code: QZ 310

Submission ReceivedDecember 10, 2013
Peer Review Double Blind
Handling Editor
Accepted January 4, 2014
Published January 20, 2014

Version of record

v1.2

Issue date

January 20, 2014

Language

English

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Article Matrices

Total Score: 141

Country: United States

Subject: Global Journal of Medical Research - K: Interdisciplinary

Authors: Amy Sun, Raymond L. Lam, Amy Harman, Anna C. Pavlick, Gary A. Herman, Lisa M. Dauffenbach, Christopher A. Kerfoot, Pearl Huang, Jonathan Cheng, Tim Demuth, Robert Iannone (PhD/Dr. count: 0)

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Publish Date: 2014 01, Wed

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