Influence Evaluation of Ocimum Sanctum Leaf Extract on Angiogenesis by using Chick Chorioallantoic Membrane (CAM) Assay

1
U. H. Shah
U. H. Shah
2
G. R. Gonjari
G. R. Gonjari
3
A. E. Patil
A. E. Patil
1 Balwant College

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Influence Evaluation of Ocimum Sanctum Leaf Extract on Angiogenesis by using Chick Chorioallantoic Membrane (CAM) Assay Banner
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Ocimum sanctum is a sacred herb of India is also known as ‘Tulsi’ or ‘Holy Basil’. There are several medicinal properties attributed to leaf, bark, root as well as seeds of O. sanctum. This holy plant is used in the present investigation to study its angiogenic efficiency. The effect of methanolic extract of O. sanctum leaves was studied by using chick chorioallantoic membrane (CAM) assay in ovo. The angiogenesis was studied after 48 hrs, 72 hrs and 96 hrs treatment of on chick CAM after day 6. The CAM was studied morphometrically and histologically. There was highly significant decrease in number of secondary and tertiary blood vessels as well as in total CAM area.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

U. H. Shah. 2015. \u201cInfluence Evaluation of Ocimum Sanctum Leaf Extract on Angiogenesis by using Chick Chorioallantoic Membrane (CAM) Assay\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 15 (GJSFR Volume 15 Issue C4): .

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GJSFR Volume 15 Issue C4
Pg. 13- 19
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification: FOR Code: 060799
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v1.2

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June 18, 2015

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English

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Ocimum sanctum is a sacred herb of India is also known as ‘Tulsi’ or ‘Holy Basil’. There are several medicinal properties attributed to leaf, bark, root as well as seeds of O. sanctum. This holy plant is used in the present investigation to study its angiogenic efficiency. The effect of methanolic extract of O. sanctum leaves was studied by using chick chorioallantoic membrane (CAM) assay in ovo. The angiogenesis was studied after 48 hrs, 72 hrs and 96 hrs treatment of on chick CAM after day 6. The CAM was studied morphometrically and histologically. There was highly significant decrease in number of secondary and tertiary blood vessels as well as in total CAM area.

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Influence Evaluation of Ocimum Sanctum Leaf Extract on Angiogenesis by using Chick Chorioallantoic Membrane (CAM) Assay

U. H. Shah
U. H. Shah Balwant College
G. R. Gonjari
G. R. Gonjari
A. E. Patil
A. E. Patil

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