Isolation and Characterization of a New Phytotoxic Molecule from Culture Fluids of Verticillium Dahliae

1
Hassan B. Lazrek
Hassan B. Lazrek
2
Hassan Laouane
Hassan Laouane
3
My Hassan Sedra
My Hassan Sedra
1 University Cadi Ayyad

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The verticillium dahliae liquid culture was extracted with butanol. The BE was able to induce necrosis of cutting stem of olive tree at 40 μg/ml the same toxicity was obtained with acetone precipitate (AP) at 50 μg/ml. The CPG profile of the fraction obtained by purification of BE on preparative TLC showed the presence of two peaks. The identification of these two compounds was based on the mass spectrum and confirmed by the data from library of mass spectra NIST (National Institute of Standards and Technology), and were assigned as two isomers: E-cinnamyl acetate (major product, VdT) and Z-cinnamyl acetate (minor product).

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Funding

No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Hassan B. Lazrek. 2013. \u201cIsolation and Characterization of a New Phytotoxic Molecule from Culture Fluids of Verticillium Dahliae\u201d. Global Journal of Science Frontier Research - B: Chemistry GJSFR-B Volume 13 (GJSFR Volume 13 Issue B7): .

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GJSFR Volume 13 Issue B7
Pg. 37- 42
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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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December 25, 2013

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The verticillium dahliae liquid culture was extracted with butanol. The BE was able to induce necrosis of cutting stem of olive tree at 40 μg/ml the same toxicity was obtained with acetone precipitate (AP) at 50 μg/ml. The CPG profile of the fraction obtained by purification of BE on preparative TLC showed the presence of two peaks. The identification of these two compounds was based on the mass spectrum and confirmed by the data from library of mass spectra NIST (National Institute of Standards and Technology), and were assigned as two isomers: E-cinnamyl acetate (major product, VdT) and Z-cinnamyl acetate (minor product).

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Isolation and Characterization of a New Phytotoxic Molecule from Culture Fluids of Verticillium Dahliae

Hassan Laouane
Hassan Laouane
My Hassan Sedra
My Hassan Sedra
Hassan B. Lazrek
Hassan B. Lazrek University Cadi Ayyad

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