Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

Zahra Zahid Piracha

Contact

Umar Saeed

Contact

Aqsa Khurshid

Contact

Waqas Nasir Chaudhary

Contact

α National University of Sciences and Technology

Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa

Article Fingerprint

ReserarchID

2978Q

Isolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa Banner

AI TAKEAWAY

Connecting with the Eternal Ground

Abstract

Pseudomonas aeruginosa is an opportunistic pathogen, frequently associated with nosocomial infections worldwide. Multiple drug resistance has been reported in previous studies against pathogenic P. aeruginosa and the biofilm which makes use of antibiotics futile. Bacteriophages specific for P. aeruginosa can prove to be a new therapeutic approach for controlling infections and biolfilm contamination against this pathogen. The aim of our study was to isolate and partially characterize virulent phage specific for P. aeruginosa from sewage water. Different parameters of which make phages as suitable candidate towards future therapeutics were also investigated. Phages having lytic life cycle, high burst size and thermally more stable proposed themselves as effective therapeutic candidates. In this study, a virulent phage was isolated from sewage water having burst size of 1036 and latent period of 21 minutes. This phage has narrow host range and shows the remarkable thermal resistance and is viable up to 60 ºC.

References

34 Cites in Article

Reference Format

V Kenneth,M Rolston,P Gerald,M Bodey (1992). Pseudomonas aeruginosa Infection in Cancer Patients.
K Ranjan,Neelima Ranjan,Satish Bansal,D Arora (2010). Prevalence of Pseudomonas aeruginosa in Post-operative Wound Infection in a Referral Hospital in Haryana, India.
J Khan,Z Iqbal,S Rahman,K Farzana,A Khan (2008). Prevalence and resistance pattern of Pseudomonas aeruginosa against various antibiotics.
K Todar (2011). Todar's online book of bacteriology.
M Souli,I Galani,H Giamarellou (2008). Emergence of extensively drug-resistant and pandrug-resistant gram-negative bacilli in Europe.
Farhat Ullah,Salman Malik,Jawad Ahmed (2012). Antimicrobial susceptibility and ESBL prevalence in Pseudomonas aeruginosa isolated from burn patients in the North West of Pakistan.
Tanya Strateva,Daniel Yordanov (2009). Pseudomonas aeruginosa – a phenomenon of bacterial resistance.
Jason Clark,John March (2006). Bacteriophages and biotechnology: vaccines, gene therapy and antibacterials.
Juan Hermoso,José García,Pedro García (2007). Taking aim on bacterial pathogens: from phage therapy to enzybiotics.
T Hausler (2007). Viruses vs. Superbugs: a solution to antibiotics or crisis.
L Bren (2007). Bacteria-eating virus approved as food additive.
Biswajit Biswas,Sankar Adhya,Paul Washart,Brian Paul,Andrei Trostel,Bradford Powell,Richard Carlton,Carl Merril (2002). Bacteriophage Therapy Rescues Mice Bacteremic from a Clinical Isolate of Vancomycin-Resistant Enterococcus faecium.
K Ashelford,S Norris,J Fry,M Bailey,M Day (2000). Seasonal population dynamics and interactions of competing bacteriophages and their host in the rhizosphere.
Sherwood Casjens (2008). Diversity among the tailed-bacteriophages that infect the Enterobacteriaceae.
Paula Watnick,Roberto Kolter (2000). Biofilm, City of Microbes.
P Gilbert,J Das,I Foley (1997). Biofilm susceptibility to antimicrobials.
George Garrity,Julia Bell,Timothy Lilburn (2005). Pasteurellales ord. nov..
M Clokie,A Kropinski (2008). Bacteriophages.
M Adams (1959). Bacteriophage.
E Ellis,M Delbruck (1939). The growth of bacteriophage.
V Suarez,S Moineau,J Reinheimer,A Quiberoni,M Zimmer,S Scherer,M Loesnner (2002). Genomic analysis of Clostridium perfringes bacteriophage phi 3626 which integrate into guaA and possibly affect sporulation.
Ebbing Lautenbach,Marie Synnestvedt,Mark Weiner,Warren Bilker,Lien Vo,Jeff Schein,Myoung Kim (2010). Imipenem Resistance in Pseudomonas aeruginosa Emergence, Epidemiology, and Impact on Clinical and Economic Outcomes.
F Richards (1969). The genetics of bacteria and their viruses.
I Wang,D Dykhuizen,L Slobodkin (1996). The evolution of phage lysis timing.
R Moldovan,Chapman,M Quiston,X Wu (2007). Kinetics of phage adsorption.
G Rice,K Stedman,J Synder,B Weidenheft (2001). Viruses from extreme l thermal environments.
Se Park,Ichiro Shimamura,Minoru Fukunaga,Koh-Ichiro Mori,Toshihiro Nakai (2000). Isolation of Bacteriophages Specific to a Fish Pathogen, Pseudomonas plecoglossicida , as a Candidate for Disease Control.
Umar Saeed,Y Waheed,S Manzoor,M Ashraf (2013). Identification of novel silent HIV propagation routes in Pakistan.
Umar Saeed,Yasir Waheed,Muhammad Ashraf (2014). Hepatitis B and hepatitis C viruses: a review of viral genomes, viral induced host immune responses, genotypic distributions and worldwide epidemiology.
U Saeed,N Jalal (2013). The Nature, Pathogenesis and Prevention of Metabolic Disorders.
U Saeed,N Nawaz,Y Waheed,N Chaudry,H Bhatti,S Urooj (2012). In silico identification of BIM-1 (2methyl-1H-indol-3-yl) as a potential therapeutic agent against elevated protein kinase C beta associated diseases.
U Saeed,Y Waheed,A Anwar,T Hussain,K Naeem,N Nawaz (2011). In-Silico Binding of ATP to Protein Kinase C Delta: Insights into the Structure and Active Site.
U Saeed,N Jalal,M Ashraf (2012). Roles of Cyclin Dependent Kinase and Cdk-Activating Kinase in Cell Cycle Regulation: Contemplation of Intracellular Interactions and Functional Characterization.
U Saeed,Y Waheed,A Anwar,M Ashraf (2013). Structural Characterization of Cytochrome P450 1A2; Insights into Insilico Inhibition via Alpha-naphthoflavone.

Download References

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Zahra Zahid Piracha. 2014. \u201cIsolation and Partial Characterization of Virulent Phage Specific against Pseudomonas Aeruginosa\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 14 (GJMR Volume 14 Issue C1): .

More Citation Formats

Select Citation Style:

Download Citation

Download Article

GJMR Volume 14 Issue C1
Pg. 1- 8

Explore Journals Explore Volume Read This Issue

Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

isolation partial characterization phage pseudomonas aeruginosa

Submission ReceivedDecember 7, 2013
Peer Review Double Blind
Handling Editor
Accepted January 2, 2014
Published January 15, 2014

Version of record

v1.2

Issue date

March 8, 2014

Language

Experiance in AR

Explore published articles in an immersive Augmented Reality environment. Our platform converts research papers into interactive 3D books, allowing readers to view and interact with content using AR and VR compatible devices.

View in VR

Read in 3D

Your published article is automatically converted into a realistic 3D book. Flip through pages and read research papers in a more engaging and interactive format.

View in 3D

Article Matrices

Total Score: 104

Country: Pakistan

Subject: Global Journal of Medical Research - C: Microbiology & Pathology

Authors: Zahra Zahid Piracha, Umar Saeed, Aqsa Khurshid, Waqas Nasir Chaudhary (PhD/Dr. count: 0)

View Count (all-time): 138

Total Views (Real + Logic): 4749

Total Downloads (simulated): 2445

Publish Date: 2014 03, Sat

Monthly Totals (Real + Logic):

Month 1: 36 views
Month 2: 33 views
Month 3: 49 views
Month 4: 61 views
Month 5: 45 views
Month 6: 47 views
Month 7: 39 views
Month 8: 27 views
Month 9: 53 views
Month 10: 16 views
Month 11: 48 views
Month 12: 25 views
Month 13: 34 views
Month 14: 22 views
Month 15: 34 views
Month 16: 25 views
Month 17: 45 views
Month 18: 32 views
Month 19: 17 views
Month 20: 29 views
Month 21: 13 views
Month 22: 34 views
Month 23: 18 views
Month 24: 22 views
Month 25: 38 views
Month 26: 29 views
Month 27: 50 views
Month 28: 46 views
Month 29: 43 views
Month 30: 11 views
Month 31: 32 views
Month 32: 44 views
Month 33: 33 views
Month 34: 35 views
Month 35: 38 views
Month 36: 25 views
Month 37: 33 views
Month 38: 39 views
Month 39: 23 views
Month 40: 31 views
Month 41: 48 views
Month 42: 30 views
Month 43: 18 views
Month 44: 43 views
Month 45: 36 views
Month 46: 25 views
Month 47: 35 views
Month 48: 32 views
Month 49: 34 views
Month 50: 26 views
Month 51: 24 views
Month 52: 28 views
Month 53: 43 views
Month 54: 23 views
Month 55: 39 views
Month 56: 34 views
Month 57: 22 views
Month 58: 38 views
Month 59: 38 views
Month 60: 23 views
Month 61: 30 views
Month 62: 24 views
Month 63: 43 views
Month 64: 22 views
Month 65: 29 views
Month 66: 43 views
Month 67: 36 views
Month 68: 37 views
Month 69: 36 views
Month 70: 18 views
Month 71: 21 views
Month 72: 42 views
Month 73: 27 views
Month 74: 37 views
Month 75: 43 views
Month 76: 33 views
Month 77: 42 views
Month 78: 35 views
Month 79: 34 views
Month 80: 36 views
Month 81: 31 views
Month 82: 41 views
Month 83: 44 views
Month 84: 18 views
Month 85: 13 views
Month 86: 25 views
Month 87: 28 views
Month 88: 41 views
Month 89: 23 views
Month 90: 13 views
Month 91: 22 views
Month 92: 41 views
Month 93: 24 views
Month 94: 34 views
Month 95: 38 views
Month 96: 47 views
Month 97: 42 views
Month 98: 39 views
Month 99: 37 views
Month 100: 22 views
Month 101: 24 views
Month 102: 32 views
Month 103: 20 views
Month 104: 34 views
Month 105: 31 views
Month 106: 12 views
Month 107: 19 views
Month 108: 34 views
Month 109: 31 views
Month 110: 25 views
Month 111: 40 views
Month 112: 25 views
Month 113: 43 views
Month 114: 35 views
Month 115: 11 views
Month 116: 26 views
Month 117: 38 views
Month 118: 29 views
Month 119: 30 views
Month 120: 13 views
Month 121: 30 views
Month 122: 24 views
Month 123: 20 views
Month 124: 40 views
Month 125: 32 views
Month 126: 28 views
Month 127: 24 views
Month 128: 47 views
Month 129: 33 views
Month 130: 28 views
Month 131: 26 views
Month 132: 36 views
Month 133: 26 views
Month 134: 35 views
Month 135: 42 views
Month 136: 27 views
Month 137: 21 views
Month 138: 42 views
Month 139: 23 views
Month 140: 28 views
Month 141: 31 views
Month 142: 14 views
Month 143: 44 views
Month 144: 28 views
Month 145: 54 views
Month 146: 37 views

Total Views: 4749

Total Downloads: 2445

2026 Trends

Published Article

Our website is actively being updated, and changes may occur frequently. Please clear your browser cache if needed. For feedback or error reporting, please email [email protected]