Isolation, Selection and Antimicrobial Activity of Actinomycetes from Mangrove Soil of Can Gio Forest, Hochiminh City, Vietnam

Isolation, Selection and Antimicrobial Activity of Actinomycetes from Mangrove Soil of Can Gio Forest, Hochiminh City, Vietnam

Nguyen Tuan Hai

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Cao Ngoc Diep

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Saigon University

Isolation, Selection and Antimicrobial Activity of Actinomycetes from Mangrove Soil of Can Gio Forest, Hochiminh City, Vietnam

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Abstract

The Can Gio Mangrove is a Biosphere Reserve of UNESCO since 2000 and it is also a well-known example of “mangrove afforestation and reforestation area”. A total of 63 actinomycetes were isolated from 25 samples of 9 different sites in mangrove forest soil Can Gio, HoChiMinh city, Vietnam. Almost their colonies have round-shaped; milky, white clear and yellow, entire or loabate margin; diameter size of these colonies varied from 0.2 to 3.0 mm. Twenty-nine of 63 tested isolates could produce antimicrobial active metabolites inhibiting at least one of the tested pathogens and 9 isolates were selected for 16S rDNA sequencing. The result showed that genus Streptomyces (8 isolates) and other genera including 1 isolates were Gram-negative bacteria (Stenotrophomonas). The antimicrobial activity and the amplifying genes coding for polyketide synthetase (PKS) and nonribosomal peptide synthetase (NRPS) showed that 8 strains had broad-spectrum antimicrobial activity, mainly against gram-positive bacteria as Bacillus cereus and Staphylococcus aureus.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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How to Cite This Article

Nguyen Tuan Hai. 2021. \u201cIsolation, Selection and Antimicrobial Activity of Actinomycetes from Mangrove Soil of Can Gio Forest, Hochiminh City, Vietnam\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 21 (GJSFR Volume 21 Issue C4).

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GJSFR Volume 21 Issue C4

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification FOR Code: 060599

Submission ReceivedJuly 14, 2021
Peer Review Double Blind
Handling Editor
Accepted August 6, 2021
Published August 19, 2021

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October 4, 2021

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