Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

olabisi_olufunmilayo_ogunrinola

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Olabisi Olufunmilayo Ogunrinola

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Olusegun Omolade Fajana

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Olaseni Pascal Oresanya

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Lagos State University

Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

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Abstract

The plant latex consist of biologically active compounds useful for diverse health benefits and could be a potential source of unique proteases. Proteases play key roles in the regulation of biological processes in plants and currently have become a vital part of the food and feed industry. In order to understand the underlying mechanisms of action of unique plant latex, the present study isolate and characterized a protease enzyme from the crude latex of plantain plant. Crude latex collected from different parts (leaves, stem, trunk and branches) of the plantain plant was centrifuge to separate the supernatant from any particles. The supernatant was used as the crude latex extract for the research. A protease enzyme was isolated from the crude latex extract and the effect of pH, temperature, incubation time, kinetic study of the protease activity as well as protein were determined spectrophoto-metrically. The enzyme protease was found to have a 9.84 mg/ml of protein with a specific activity of 3.06 unit/mg protein respectively.

References

39 Cites in Article

Reference Format

Kotaro Konno (2011). Plant latex and other exudates as plant defense systems: Roles of various defense chemicals and proteins contained therein.
Lucía Feijoo-Siota,Tomás Villa (2011). Native and Biotechnologically Engineered Plant Proteases with Industrial Applications.
Jeana Macalood,Helen Vicente,Renato Boniao,Jessie Gorospe,Elnor Roa (2013). Chemical Analysis of <i>Carica papaya</i> L. Crude Latex.
M Mazorra-Manzano,J Ramírez-Suarez,R Yada (2018). Plant proteases for bioactive peptides release: A review.
S Venkatesha,R Rajaiah,B Vishwanath (2016). Hemostatic interference of plant latex proteases.
Ravi Upadhyay (2011). Plant latex: A natural source of pharmaceuticals and pesticides.
Amog Urs,V Manjuprasanna,G Rudresha,M Yariswamy,B Vishwanath (2017). Plant Latex Proteases: Natural Wound Healers.
Boris Turk (2006). Targeting proteases: successes, failures and future prospects.
Jeremy Berg,John Tymoczko,Lubert Stryer (2007). Stryer Biochemie.
J Vionoth,S Murugan,C Stalin (2014). Optimization of alkaline proteases production and its fibrinolytic activity from the bacterium Pseudomonas fluorescens isolated from fish waste discharged soil.
V Dubey,M Pande,B Singh (2007). Coronaviruses papain-like proteases and their inhibitors.
Charles Craik,Michael Page,Edwin Madison (2011). Proteases as therapeutics.
Janet Ramundo,Mikel Gray (2008). Enzymatic Wound Debridement.
A Li,D Ai-Yun,C (2007). Purification and characterization of two thermostable proteases from the thermophilic fungus Chaetomium thermophilum.
Abhay Singh,Anil Shukla,M Jagannadham,Vikash Dubey (2010). Purification of a novel cysteine protease, procerain B, from Calotropis procera with distinct characteristics compared to procerain.
Ritu Tomar,Reetesh Kumar,M Jagannadham (2008). A Stable Serine Protease, Wrightin, from the Latex of the Plant Wrightia tinctoria (Roxb.) R. Br.: Purification and Biochemical Properties.
André Domsalla,Matthias Melzig (2008). Occurrence and Properties of Proteases in Plant Latices.
Robert Harrison,Bryony Bonning (2010). Proteases as Insecticidal Agents.
R Upadhyay (2013). Bio-efficacy of latex extracts from plant species Thevetia nerifolia, and Artocarpus heterophyllus, Ficus glomerata and Calotropis procera on survival, feeding, development and reproductive behavior of Spodoptera litura (F.) Noctuidae: Lepidoptera.
Cecília Antão,F Malcata (2005). Plant serine proteases: biochemical, physiological and molecular features.
Vadim Beilinson,Oleksandr Moskalenko,Donald Livingstone,Sergei Reverdatto,Rudolf Jung,Niels Nielsen (2002). Two subtilisin‐like proteases from soybean.
O Awoyinka,S Shokunbi (2005). Comparative Studies Of The Proteolytic And The Milk Clotting Activities In The \'Latex\' Of Three Selected Plants.
A Shaibu,E Maji,M Ogburia (2012). Yield evaluation of plantain and banana landraces and hybrids in humid agro ecological zone of Nigeria.
A Adeolu,D Enesi (2013). Assessment of proximate, mineral, vitamin and phytochemical compositions of plantain (Musa paradisiaca) bract -an agricultural waste.
Ennis Layne (1957). [73] Spectrophotometric and turbidimetric methods for measuring proteins.
Alastair Aitken,Michèle Learmonth (2009). Protein Determination by UV Absorption.
P Jenssen,K Peek,H Morgan (1994). Effect of culture conditions on the production of an extracellular proteinase by Thermus sp. Rt41A.
O Raimi,M Kappo,O Fajana (2010). Supplementary file 1..
O Raimi,B Elemo,A Fatai (2011). Isolation and partial characterization of a protease enzyme from Thaumatococcus daniellii waste.
Mala Rao,Aparna Tanksale,Mohini Ghatge,Vasanti Deshpande (1998). Molecular and Biotechnological Aspects of Microbial Proteases.
Anurag Agrawal,Kotaro Konno (2009). Latex: A Model for Understanding Mechanisms, Ecology, and Evolution of Plant Defense Against Herbivory.
N Sevinc,E Demirkan (2011). Microbial Characterization and Optimization of Protease Production by Bacillus SP. Isolated from Soil.
Aleksandra Shedoeva,David Leavesley,Zee Upton,Chen Fan (2019). Wound Healing and the Use of Medicinal Plants.
Abdul Razzaq,Sadia Shamsi,Arfan Ali,Qurban Ali,Muhammad Sajjad,Arif Malik,Muhammad Ashraf (2019). Microbial Proteases Applications.
A Sumantha,C Sandhya,G Szakacs (2005). Production and partial purification of a neutral metalloprotease by fungal mixed substrate fermentation.
E Tondo,F Lakus,F Oliveira,A Brandelli (2004). Identification of heat stable protease of Klebsiella oxytoca isolated from raw milk.
Girijesh Patel,Ashish Kawale,Ashwani Sharma (2012). Purification and physicochemical characterization of a serine protease with fibrinolytic activity from latex of a medicinal herb Euphorbia hirta.
M Dahot,A Sheikh (2005). Neutral protease activity in the crude extract of cotton (Gossypium hirsutum) seeds.
T Sharma,P Dash,D Das (2014). Kinetic and thermodynamic studies of purified protein isolated from Euphorbia tirucalli latex.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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How to Cite This Article

olabisi_olufunmilayo_ogunrinola. 2021. \u201cKinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant\u201d. Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 21 (GJSFR Volume 21 Issue C1).

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GJSFR Volume 21 Issue C1

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification FOR Code: 060199

Submission ReceivedJanuary 23, 2021
Peer Review Double Blind
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Accepted February 19, 2021
Published March 4, 2021

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March 8, 2021

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