Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

Olabisi Olufunmilayo Ogunrinola; Olusegun Omolade Fajana; Olaseni Pascal Oresanya

Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

Olabisi Olufunmilayo Ogunrinola * §

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Olusegun Omolade Fajana

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Olaseni Pascal Oresanya

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Lagos State University

Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant

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Abstract

The plant latex consist of biologically active compounds useful for diverse health benefits and could be a potential source of unique proteases. Proteases play key roles in the regulation of biological processes in plants and currently have become a vital part of the food and feed industry. In order to understand the underlying mechanisms of action of unique plant latex, the present study isolate and characterized a protease enzyme from the crude latex of plantain plant. Crude latex collected from different parts (leaves, stem, trunk and branches) of the plantain plant was centrifuge to separate the supernatant from any particles. The supernatant was used as the crude latex extract for the research. A protease enzyme was isolated from the crude latex extract and the effect of pH, temperature, incubation time, kinetic study of the protease activity as well as protein were determined spectrophoto-metrically. The enzyme protease was found to have a 9.84 mg/ml of protein with a specific activity of 3.06 unit/mg protein respectively.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

Olabisi Olufunmilayo Ogunrinola, Olusegun Omolade Fajana, Olaseni Pascal Oresanya. 2021. "Kinetic Characterization of a Protease Isolated from Crude Fresh Latex of Plantain (Musa Paradisiaca) Plant". Global Journal of Science Frontier Research - C: Biological Science GJSFR-C Volume 21 (GJSFR Volume 21 Issue C1).

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GJSFR Volume 21 Issue C1

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-C Classification FOR Code: 060199

Submission ReceivedJanuary 23, 2021
Peer Review Double Blind
Handling Editor
Accepted February 19, 2021
Published March 4, 2021

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v1.2

Issue date

March 8, 2021

Language

English

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