Laboratory-scale bioremediation experiments on diesel and Polycyclic aromatic hydrocarbons contaminated soils

Dr. Seyed Mostafa khezri

Laboratory-scale bioremediation experiments on diesel and Polycyclic aromatic hydrocarbons contaminated soils

Dr. Seyed Mostafa khezri * §

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Islamic Azad University, Tehran, Iran

Laboratory-scale bioremediation experiments on diesel and Polycyclic aromatic hydrocarbons contaminated soils

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Abstract

Laboratory investigations were performed to estimate the potential of bioslurry for bioremediation of PAHs and TPH-contaminated soil of Azimabad region in the south of Tehran refinery plant. The soil slurry-Sequencing Batch Reactor (SS-SBR) consisted of covered 8L Plexiglas vessel with a working volume of 6L. The reactor was equipped with a 400 rpm speed mixer. Oxygen was supplied through a fine-bubble diffuser at the bottom. A granular activated carbon trap as used to collect volatile organics in effluent air, fill period was relatively instantaneous, and Draw period lasted approximately 1 minute so the recent period comprised essentially the entire cycle time. In draw period a fraction of slurry (10%) was removed from period a fraction of slurry (10%) was removed from the SS-SBR weekly and it replaced with untreated slurry. This volumetric replacement volume of slurry (the recycle fraction) remained in the reactor to provide acclimated microorganism for the next batch of untreated slurry. The soil used in slurry studies was poorly graded sand with clay (SP-SC) which contaminated with approximately 67500 mg/kg TPH and 500mg/kg PAHS and passed through a sieve with an opening diameter of 0.5mm tap water was added to produce solids concentration of 10% (0.1kg dry soil/l slurry). Ammonias nitrate and phosphate were added to provide a C: N: P ratio of approximately 60:2:1 process performance .The TPH concentrations were determined by Gas chromatography with Flame Ionization Detector (GC-FID) after ultrasonic extraction the PAHS concentrations were determined with both of High performance liquid chromatography (HPLC) an GC-FID after silica-gel clean up. The results have shown high overall removal efficiency for TPH close to 96%. whereas PAHs were not detected at the end of each cycles.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Dr. khezri. 2011. "Laboratory-scale bioremediation experiments on diesel and Polycyclic aromatic hydrocarbons contaminated soils". Global Journal of Research in Engineering, Global Journal of Research in Engineering - B: Automotive Engineering GJRE-B Volume 11 (GJRE Volume 11 Issue B5).

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GJRE Volume 11 Issue B5

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Journal Specifications

Crossref Journal DOI 10.17406/gjre

Print ISSN 0975-5861

e-ISSN 2249-4596

Keywords

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Classification

GJRE-C Classification FOR Code: 030301

090401

Submission ReceivedApril 19, 2011
Peer Review Double Blind
Handling Editor
Accepted May 13, 2011
Published May 28, 2011

Version of record

v1.2

Issue date

September 7, 2011

Language

English

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Article Matrices

Total Score: 88

Country: Iran

Subject: Global Journal of Research in Engineering

Authors: Dr. Seyed Mostafa khezri,Seyed Hamidreza Fatemi, Saber Hasanlou (PhD/Dr. count: 1)

View Count (all-time): 301

Total Views (Real + Logic): 1685

Total Downloads (simulated): 116

Publish Date: 2011 05, Sat

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Total Views: 1.7K

Total Downloads: 116

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