Lipid Peroxidation during the Cryopreservation Process of Porcine Spermatozoa

J. Simoni+ S.D. Prien

Lipid Peroxidation during the Cryopreservation Process of Porcine Spermatozoa

J. Simoni+ S.D. Prien * §

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Lipid Peroxidation during the Cryopreservation Process of Porcine Spermatozoa

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Abstract

The potential advantages of sperm cryopreservation have not been fully accomplished due to the limiting detrimental effects the freezing process has on sperm structure and composition. Previous studies have suggested that cells suffer lipid peroxidation damage during the cryopreservation process, specifically indicating the damage results from mechanical stress during the preparatory and freezing processes. In this present study, sperm samples were analyzed for lipid stability throughout sample processing through evaluations for lipid peroxidation and lipid free radical concentration. Our analysis was completed in three experiments. In Exp. #1, lipid stability levels were evaluated from five separate boar ejaculates frozen using three different freezing methods to compare cryopreservation techniques. In Exp. #2, lipid peroxidation amounts for fresh post-ejaculate and albumin extended boar samples were compared. Experiment #3 involved evaluations of the semen processing to examine sample and seminal fluid alterations. Samples tested from the freezing protocol included fresh, extended, addition of a wash buffer, cooling to 17 ºC, centrifugation, addition of two egg-yolk extenders, cooling to 5 ºC and post-thaw values. Though there was no difference between the three freezing treatments, significant differences were noted between the fresh and extended samples (P < 0.001).

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29 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

J. Prien. 2014. "Lipid Peroxidation during the Cryopreservation Process of Porcine Spermatozoa". Global Journal of Medical Research, Global Journal of Medical Research - E: Gynecology & Obstetrics GJMR-E Volume 14 (GJMR Volume 14 Issue E4).

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GJMR Volume 14 Issue E4

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

cryopreservation lipid peroxidation porcine sperm

Classification

GJMR-E Classification NLMC Code: WO 510

WJ 834

Submission ReceivedDecember 10, 2013
Peer Review Double Blind
Handling Editor
Accepted December 31, 2013
Published January 15, 2014

Version of record

v1.2

Issue date

October 4, 2014

Language

English

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Total Score: 173

Country: United States

Subject: Global Journal of Medical Research

Authors: H.A. Goolsby, G. Simoni, J. Simoni+ S.D. Prien (PhD/Dr. count: 0)

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