Micro-Satellite DNA Markers Associated with Resistance to Trembling Disease in Chinese Mitten Crab (Ericheirsinensis)

1
Ali Sserwadda
Ali Sserwadda
2
Tian-Hao Dai
Tian-Hao Dai
3
Yuanchao Ma
Yuanchao Ma
4
Yacheng Hu
Yacheng Hu
5
Huai Shun Shen
Huai Shun Shen
1 Nanjing Agricultural University

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A total of 48 individuals of the Chinese mitten crab (Eriocheir sinensis) were used to study the association between micro-satellite DNA markers and their resistance to the trembling disease. The crabs used in this study were collected from an aquatic breeding farm in Yandu District, Yancheng city, Jiangsu Province in China. Twenty four of the crabs were collected from a pond heavily infested with trembling disease and these manifested clear signs of the disease while the remaining 24 crabs were collected from a pond free of the disease and these were all healthy. The Shannon Diversity Index was used to analyze the association of the individual marker alleles with resistance to the trembling disease. The results showed that the mean number of alleles (Na), the mean number of effective alleles (Ne), mean observed heterozygosity (Ho), mean expected heterozygosity (He), mean polymorphic information content (PIC) and mean Shannon index (I)in the individual populations were 4.2, 3.2, 0.6628, 0.6466,0.6221 and 1.2, respectively.

Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

Ali Sserwadda. 2017. \u201cMicro-Satellite DNA Markers Associated with Resistance to Trembling Disease in Chinese Mitten Crab (Ericheirsinensis)\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 17 (GJSFR Volume 17 Issue D2): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-D Classification: FOR Code: 079999
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v1.2

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April 26, 2017

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English

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A total of 48 individuals of the Chinese mitten crab (Eriocheir sinensis) were used to study the association between micro-satellite DNA markers and their resistance to the trembling disease. The crabs used in this study were collected from an aquatic breeding farm in Yandu District, Yancheng city, Jiangsu Province in China. Twenty four of the crabs were collected from a pond heavily infested with trembling disease and these manifested clear signs of the disease while the remaining 24 crabs were collected from a pond free of the disease and these were all healthy. The Shannon Diversity Index was used to analyze the association of the individual marker alleles with resistance to the trembling disease. The results showed that the mean number of alleles (Na), the mean number of effective alleles (Ne), mean observed heterozygosity (Ho), mean expected heterozygosity (He), mean polymorphic information content (PIC) and mean Shannon index (I)in the individual populations were 4.2, 3.2, 0.6628, 0.6466,0.6221 and 1.2, respectively.

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Micro-Satellite DNA Markers Associated with Resistance to Trembling Disease in Chinese Mitten Crab (Ericheirsinensis)

Ali Sserwadda
Ali Sserwadda Nanjing Agricultural University
Tian-Hao Dai
Tian-Hao Dai
Yuanchao Ma
Yuanchao Ma
Yacheng Hu
Yacheng Hu
Huai Shun Shen
Huai Shun Shen

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