Minor Groove Binder Probe Real-Time RT-PCR for Detection of Foot-and-Mouth Disease Virus in Egypt

Hany I Abu-Elnaga

Minor Groove Binder Probe Real-Time RT-PCR for Detection of Foot-and-Mouth Disease Virus in Egypt

Hany I Abu-Elnaga *

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Minor Groove Binder Probe Real-Time RT-PCR for Detection of Foot-and-Mouth Disease Virus in Egypt

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Abstract

Shorter, more specific minor groove binders (MGBs) probes are dsDNA-binding agents attached to the 3′ end of Taq Man probes that could be designed strictly to the invariant region. Application and assessing of a new trend for viral detection in Egypt depending on MGB probe real-time RT-PCR (rRT-PCR) applied on local FMDV serotypes O, A, and SAT2. Moreover, FMDV O was detected using two serotype specific primer sets by SYBR Green real-time RT-PCR assaying rapid formats. The limit of detection of diluted RNAs using MGB probe rRT-PCR assay reached to ≤ 6 FG/ul. Besides, the high specificity of it was clear. In contrary, the employing of FMDV O specific primer pairs in SYBR Green real-time RT-PCR showed less sensitivity and specificity, particularly one of them displayed poor performance illustrating important cause of the false negative results in the conventional PCR. Lastly, the local financial cost of MGB probe is considered the obvious hinder in my country.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Hany I Abu-Elnaga. 2019. "Minor Groove Binder Probe Real-Time RT-PCR for Detection of Foot-and-Mouth Disease Virus in Egypt". Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 19 (GJMR Volume 19 Issue G1).

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GJMR Volume 19 Issue G1

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-G Classification NLMC Code:QW 70

Submission ReceivedDecember 13, 2018
Peer Review Double Blind
Handling Editor
Accepted January 3, 2019
Published January 15, 2019

Version of record

v1.2

Issue date

March 9, 2019

Language

English

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Total Score: 141

Country: Egypt

Subject: Global Journal of Medical Research

Authors: Hany I Abu-Elnaga (PhD/Dr. count: 0)

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Publish Date: 2019 01, Tue

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