Molecular Characterization of Recent Isolates of BEF Virus in Egypt

Hiam M. Fakhry

Molecular Characterization of Recent Isolates of BEF Virus in Egypt

Hiam M. Fakhry * §

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§ Veterinary Serum and Vaccine Research Institute,

Molecular Characterization of Recent Isolates of BEF Virus in Egypt

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Abstract

The present work studied an outbreak of bovine ephemeral fever (BEF) at El-Salhia dairy farm during July and August 2015. The herd was consisting of 1600 previously vaccinated cows. Clinical examination of the herd revealed clinical signs lead to suspect infection with bovine ephemeral fever as fever; harried respiration; lameness and recumbency in 103 cows. Serum samples, buffy coats and blood plasma samples from the diseased cows were used for identification and characterization of recent isolates of BEFV. The 103 animals were found to have non-protective low titers of BEF antibodies (≤2-8) as tested by serum neutralization test (SNT). Trials of virus isolation in baby mice brain and BHK21 cell culture from buffy coat and blood plasma samples revealed specific signs in inoculated mice (paralysis of the limbs, nervous symptoms, and death within 3-4 days post inoculation), and specific cytopathic effect in BHK-21 cell culture. Virus identification using virus neutralization tests (VNT) and direct fluorescent antibody technique (FAT) confirmed the presence of BEFV. Also, PCR, sequencing analysis and phylogenetic tree showed that the obtained isolate is closely related to Egypt-2005 strain. These findings indicated that BEF still causes a risk to the cattle industry in Egypt although there is an effective vaccine used against the disease. So much more studies should be conducted on the risk factors of the disease.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Hiam Fakhry. 2018. "Molecular Characterization of Recent Isolates of BEF Virus in Egypt". Global Journal of Medical Research, Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 18 (GJMR Volume 18 Issue G2).

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GJMR Volume 18 Issue G2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

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Classification

GJMR-G Classification NLMC Code: WA 360

Submission ReceivedDecember 16, 2017
Peer Review Double Blind
Handling Editor
Accepted January 4, 2018
Published January 15, 2018

Version of record

v1.2

Issue date

September 29, 2018

Language

English

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Article Matrices

Total Score: 148

Country: Egypt

Subject: Global Journal of Medical Research

Authors: Albehwar, A.M., Wafaa, M. El-Neshwy, Hiam, M. Fakhry, Hemmat, S. El-Emam (PhD/Dr. count: 0)

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