Moringa Oleifera Seed Protein Hydrolysates Inhibit Haemoglobin Glycosylation and α-Glucosidase Activity in-Vitro

Moringa Oleifera Seed Protein Hydrolysates Inhibit Haemoglobin Glycosylation and α-Glucosidase Activity in-Vitro

Augustine Olusegun Olusola

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Oluwafemi Emmanuel Ekun

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Adekunle Ajasin University

Moringa Oleifera Seed Protein Hydrolysates Inhibit Haemoglobin Glycosylation and α-Glucosidase Activity in-Vitro

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Abstract

In recent times, the biological activities of enzymatic digests of plant and animal proteins have been investigated and have been shown to exhibit multidirectional effects against key enzymes involved in the pathophysiology of a number of diseases. The present study evaluated the inhibitory effects of M.oleifera seed protein hydrolysates on haemoglobin glycosylation and α-glucosidase. Proteins were hydrolyzed using the enzymes pepsin, trypsin, papain and chymotrypsin. The resulting hydrolysates were evaluated for inhibitory activities against non-enzymatic haemoglobin glycosylation as well as αglucosidase. Peptic and chymotrypsin hydrolysates demonstrated the best inhibitory effects against hemoglobin glycosylation, while chymotryptic and tryptic hydrolysates had better α-glucosidase inhibitory activities. Kinetic data showed that the hydrolysates inhibited α-glucosidase inhibitory effects by different mechanisms, such tryptic and chymotrypsin hydrolysates indicated a competitive mode of inhibition while papain and pepsin hydrolysates displayed mixed inhibition of α-glucosidase. These results suggest that M.oleifera seed proteins contain peptides that can be harnessed to formulate peptides which could serve as novel alternatives to current therapies in the management of diabetes mellitus.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

How to Cite This Article

Augustine Olusegun Olusola. 2020. \u201cMoringa Oleifera Seed Protein Hydrolysates Inhibit Haemoglobin Glycosylation and α-Glucosidase Activity in-Vitro\u201d. Global Journal of Medical Research - B: Pharma, Drug Discovery, Toxicology & Medicine GJMR-B Volume 19 (GJMR Volume 19 Issue B3).

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GJMR Volume 19 Issue B3

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-B Classification NLMC Code: QV 138

Submission ReceivedDecember 12, 2018
Peer Review Double Blind
Handling Editor
Accepted January 4, 2019
Published January 15, 2019

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January 8, 2020

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