Nitrogen Fixing Potential of Acacia Gummifera At Different Ages Inoculated With Rhizobium Isolates

1
Fatima Zahra Lahdachi
Fatima Zahra Lahdachi
2
Laila Nassiri
Laila Nassiri
3
Jamal Ibijbijen
Jamal Ibijbijen
1 Moulay Ismail University

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Acacia gummifera is an important tree from the south-west of Morocco. It has been reported to increase the soil fertility as the other Acacia. The characterization of successful strains and the study of the potential for fixing atmospheric nitrogen was a necessary in order to succeed its establishment in the environment where it will be introduced. Growth in YMA, tolerance to stress factor (pH, salt and temperature) and resistance to metallic ions were used as phenotypic markers of isolates collected from root nodule of A. gummifera. Genotypic diversity was studied by amplification of polymorphic DNA and 16s RNA gene sequencing. The symbiosis effectiveness of 6 performed Rhizobium was evaluated using plant nodulation assay at two different ages in controlled condition.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

Fatima Zahra Lahdachi. 2019. \u201cNitrogen Fixing Potential of Acacia Gummifera At Different Ages Inoculated With Rhizobium Isolates\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 19 (GJSFR Volume 19 Issue D2): .

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Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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August 6, 2019

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Acacia gummifera is an important tree from the south-west of Morocco. It has been reported to increase the soil fertility as the other Acacia. The characterization of successful strains and the study of the potential for fixing atmospheric nitrogen was a necessary in order to succeed its establishment in the environment where it will be introduced. Growth in YMA, tolerance to stress factor (pH, salt and temperature) and resistance to metallic ions were used as phenotypic markers of isolates collected from root nodule of A. gummifera. Genotypic diversity was studied by amplification of polymorphic DNA and 16s RNA gene sequencing. The symbiosis effectiveness of 6 performed Rhizobium was evaluated using plant nodulation assay at two different ages in controlled condition.

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Nitrogen Fixing Potential of Acacia Gummifera At Different Ages Inoculated With Rhizobium Isolates

Fatima Zahra Lahdachi
Fatima Zahra Lahdachi Moulay Ismail University
Laila Nassiri
Laila Nassiri
Jamal Ibijbijen
Jamal Ibijbijen

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