Optimization of Nutrient Medium Composition for Entomopathogens Bacillus Thuringiensis Cultivation

Optimization of Nutrient Medium Composition for Entomopathogens Bacillus Thuringiensis Cultivation

Maria Boiko

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National University of Life and Environmental Sciences of Ukraine

Optimization of Nutrient Medium Composition for Entomopathogens Bacillus Thuringiensis Cultivation

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Abstract

The objective of the research was to develop an optimal liquid medium composition for bacteria strain Bacillus thuringiensis 87/3 which are the most favorable for the identifying the potential production of biologically active components. In this work we have used new entomopathogenic bacteria strain Bacillus thuringiensis var. thuringiensis (Bt H1) №87/3 selected in vitro, isolated from the larvae of leaf-eating insects natural populations of Leptinotarsa decemlineata Say (L4). Nutrient medium for cultivation of Bt 87/3 was optimized by a full-fledged experiment, according to the Box-Behnken (33) plan. Optimum concentrations were investigated in the medium of carbon source (15 g/l of glucose), nitrogen (10 g/l of corn extract) and content of phosphorus-containing inorganic salts (1,5 g/l of diammonium phosphate). When growing this strain in periodic conditions at 30°C, the number of viable cells reached 4,4×109 cells/ml. The nutrient medium that was optimized can be recommended for the cultivation of Bacillus thuringiensis 87/3 in production conditions.

References

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Maria Boiko. 2019. \u201cOptimization of Nutrient Medium Composition for Entomopathogens Bacillus Thuringiensis Cultivation\u201d. Global Journal of Science Frontier Research - D: Agriculture & Veterinary GJSFR-D Volume 18 (GJSFR Volume 18 Issue D8).

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GJSFR Volume 18 Issue D8

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-D Classification FOR Code: 050299

Submission ReceivedDecember 3, 2018
Peer Review Double Blind
Handling Editor
Accepted December 27, 2018
Published January 6, 2019

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v1.2

Issue date

January 25, 2019

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Article Matrices

Total Score: 101

Country: Ukraine

Subject: Global Journal of Science Frontier Research - D: Agriculture & Veterinary

Authors: Maria Boiko (PhD/Dr. count: 0)

View Count (all-time): 178

Total Views (Real + Logic): 2900

Total Downloads (simulated): 1368

Publish Date: 2019 01, Fri

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Total Views: 2900

Total Downloads: 1368

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