Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.

1
Arghya Sur
Arghya Sur
2
Hemontika Chakraborty
Hemontika Chakraborty
3
Arindam Basu
Arindam Basu
4
Sasanka Chakrabarti
Sasanka Chakrabarti
1 Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in vivo Senescence of Erythrocyte.

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Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte. Banner
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This study was focused to identify the link between oxidative stress and senescence of erythrocytes in vivo. To elucidate this mechanism, various modification of RBC membrane proteins and lipids were analyzed invitro, exposing them to oxidative stress and the results were compared with the changes observed in erythrocytes undergoing senescence in vivo. The other objective was to confirm the mechanism of autoantibody mediated removal of aged RBCs. Our results established that increased lipid peroxidation products, followed by the enhanced damage of RBC membrane protein caused increased RBC membrane protein carbonylation, to normal red cells exposed to the in vitro Fe 2+ & ascorbate induced oxidative stress. It was presumable that these changes were mediated by hydroxyl (ȮH) radicals. Further, similar changes were also seen in percoll gradient age fractionated high density aged RBCs.

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No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Arghya Sur. 2014. \u201cOxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.\u201d. Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 14 (GJMR Volume 14 Issue C7): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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December 24, 2014

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English

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This study was focused to identify the link between oxidative stress and senescence of erythrocytes in vivo. To elucidate this mechanism, various modification of RBC membrane proteins and lipids were analyzed invitro, exposing them to oxidative stress and the results were compared with the changes observed in erythrocytes undergoing senescence in vivo. The other objective was to confirm the mechanism of autoantibody mediated removal of aged RBCs. Our results established that increased lipid peroxidation products, followed by the enhanced damage of RBC membrane protein caused increased RBC membrane protein carbonylation, to normal red cells exposed to the in vitro Fe 2+ & ascorbate induced oxidative stress. It was presumable that these changes were mediated by hydroxyl (ȮH) radicals. Further, similar changes were also seen in percoll gradient age fractionated high density aged RBCs.

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Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.

Arghya Sur
Arghya Sur Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in vivo Senescence of Erythrocyte.
Hemontika Chakraborty
Hemontika Chakraborty
Arindam Basu
Arindam Basu
Sasanka Chakrabarti
Sasanka Chakrabarti

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