Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.

Arghya Sur; Hemontika Chakraborty; Arindam Basu

Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.

Arghya Sur * §

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Hemontika Chakraborty

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Abstract

This study was focused to identify the link between oxidative stress and senescence of erythrocytes in vivo. To elucidate this mechanism, various modification of RBC membrane proteins and lipids were analyzed invitro, exposing them to oxidative stress and the results were compared with the changes observed in erythrocytes undergoing senescence in vivo. The other objective was to confirm the mechanism of autoantibody mediated removal of aged RBCs. Our results established that increased lipid peroxidation products, followed by the enhanced damage of RBC membrane protein caused increased RBC membrane protein carbonylation, to normal red cells exposed to the in vitro Fe 2+ & ascorbate induced oxidative stress. It was presumable that these changes were mediated by hydroxyl (ȮH) radicals. Further, similar changes were also seen in percoll gradient age fractionated high density aged RBCs.

References

55 Cites in Article

Reference Format

induced peroxidation of lipids.
P Low,S Waugh,K Zinke,D Drenckhahn (1985). The role of haemoglobin denaturation and band-3 clustering in red blood cell ageing.
Masatoshi Beppu,Hisashi Ochiai,Kiyomi Kikugawa (1989). Macrophage recognition of periodate-treated erythrocytes: Involvement of disulfide formation of the erythrocyte membrane proteins.
S Mazzulla,A Schella,D Gabriele,N Baldino,S Sesti,E Perrotta,A Costabile,B De Cindio (2014). Oxidation of human red blood cells by a free radical initiator: Effects on rheological properties.
R Hernandez-Munoz,M Olguin-Martinez,I Aguilar-Delfin,L Sanchez-Sevilla,N Garcia-Garcia,M Diaz-Mufioz (2013). Oxidant status and lipid composition of erythrocyte membranes in patients with type 2 diabetes, chronic liver damage, and a combination of both pathologies.
D Mendez,M Hernaez,A Kamali,A Diez,A Puyet,J Bautista (2012). Differential carbonylation of cytoskeletal proteins in blood group O erythrocytes: potential role in protection against severe malaria.
Panagiotis Margetis,Marianna Antonelou,Ioannis Petropoulos,Lukas Margaritis,Issidora Papassideri (2009). Increased protein carbonylation of red blood cell membrane in diabetic retinopathy.
K Schluter,D Drenckhahn (1986). Co-clustering of denatured hemoglobin with band-3: Its role in binding of auto antibodies against band 3 to abnormal and aged erythrocytes.
H Campwala,J Desforges (1982). Membrane bound hemichrome in density separated cohorts of normal (AA) and sickled (SS) cells.
Paul Hochstein,Catherine Rice-Evans (1981). LIPID PEROXIDATION AND MEMBRANE ALTERATIONS IN ERYTHROCYTE SURVIVAL.
P Browne,O Shalev,R Hebbel (1998). The Molecular Pathobiology of Cell Membrane Iron: The Sickle Red Cell as a Model.
Umakant Dumaswala,Limei Zhuo,Donald Jacobsen,Sushil Jain,Katherine Sukalski (1999). Protein and lipid oxidation of banked human erythrocytes:.
Sara Rinalducci,Gian D’amici,Barbara Blasi,Lello Zolla (2012). Oxidative stress-dependent oligomeric status of erythrocyte peroxiredoxin II (PrxII) during storage under standard blood banking conditions.
L Margaritis,I Papassideri,H Imanishi,T Nakai,T Abe,T Takino (1985). Glutathione metabolism in red cell ageing.
E Hodgson,I Fridovich (1975). The interaction of bovine erythrocyte superoxide dismutase with hydrogen peroxide: inactivation of the enzyme.
B Halliwell,J Gutteridge Oxygen toxicity, oxygen radicals, transition metals and disease.
B Halliwell,J Gutteridge (1989). Free Radicals in biology and Medicine.
James May,Zhi-Chao Qu,Shalu Mendiratta (1998). Protection and Recycling of α-Tocopherol in Human Erythrocytes by Intracellular Ascorbic Acid.
M O'connell,B Halliwell,C Moorhouse,O Aruoma,H Baum,T Peters (1986). Formation of hydroxyl radicals in the presence of ferritin and haemosiderin. Is haemosiderin formation a biological protective mechanism?.
B Frei,L England,B Ames (1989). Ascorbate is an outstanding antioxidant in human blood plasma..
E Shinar,A Rachmilewitz,A Shifter,E Rahamin,P Saltman Oxidative damage to human red cells induced by copper and iron complexes in the presence of ascorbate.
H Chakraborty,P Sen,A Sur,U Chatterjee,S Chakrabarti (2003). Age-related oxidative inactivation of Na+, K+-ATPase in rat brain crude synaptosomes.
A Sur,K Pal,S Sen,S Chakrabarti (2000). Synergistic Fluoroalkyl and Electron-Rich Vinyl Units for Ionizing Radiation-Induced Cross-Linking.
J Szebeni,C Winterbourn,R Carrell (1984). Oxidative interactions between haemoglobin and membrane lipid. A liposome model.
C Winterbourn,B Mcgrath,R Carrell (1976). haemoglobins.
A Puppo,B Halliwell (1998). Formation of hydroxyl radicals from hydrogen peroxide in the presence of iron: Is haemoglobin a biological Fenton reagent?.
M Sorette,U Galili,M Clark (1014). Comparison of serum anti-band 3 and anti-Gal antibody binding to density-separated human red blood cells.
G Sambrano,S Parthasarathy,D Steinberg (1994). Recognition of oxidatively damaged erythrocytes by a macrophage receptor with specificity for oxidized low density lipo protein.
M Kay (1984). Localization of senescent cell antigen on band 3..
U Galili,E Rachmilewitz,A Peleg,I Flechner (1984). A unique natural human IgG antibody with anti-alpha-galactosyl specificity..
Y Chancerelle,J Mathieu,J Kergonou (1994). Recognition and elimination of senescent erythrocytes: Implication of antibodies specific for malonic dialdehyde-protein adducts, as demonstrated by flow cytometry.
M Beppu,A Mizukami,M Nagoya,K Kikugawa (1990). Binding of anti -band 3 autoantibody to oxidatively damaged erythrocytes.
Robert Weed (1970). The importance of erythrocyte deformability.
A Williams,D Morris (1980). The Internal Viscosity of the Human Erythrocyte May Determine Its Lifespan in Vivo.
Nurith Shaklai,Betty Frayman,Normand Fortier,Michael Snyder (1987). Crosslinking of isolated cytoskeletal proteins with hemoglobin: a possible damage inflicted to the red cell membrane.
P Jarolim,M Lahav,S Liu,J Palek (1990). Effect of hemoglobin oxidation products on the stability of red cell membrane skeletons and the associations of skeletal proteins: correlation with a release of hemin.
E Beutler,C West,K Blume (1975). The removal of leukocytes and platelets from whole blood.
G Fairbanks,Theodore Steck,D Wallach (1971). Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.
Oliverh. Lowry,Niraj. Rosebrough,A Farr,Rosej. Randall (1951). PROTEIN MEASUREMENT WITH THE FOLIN PHENOL REAGENT.
B Halliwell,J Gutteridge (1981). Formation of thiobarbituric acid-reactive-substance from deoxyribose in the presence of iron salts: The role of superoxide and hydroxyl radicals.
V Terpstra,J Vanberkel,H Ohkawa,N Oshini,K Yagi (1979). Scavenger receptors on liver Kupffer cells mediate the in vivo uptake of 53.
S Chakrabarti,R Shankar (1984). Lipid peroxidation in developing rat brain during undernutrition.
R Levine,D Garland,C Oliver,A Amici,I Climent,A Lenz,B Ahn,D Shaltiel,E Stadtman (1990). Determination of carbonyl content in oxidatively modified proteins.
Hemontika Chakraborty,Sibendra Ray,Sasanka Chakrabarti (2001). Lipid peroxidation associated protein damage in rat brain crude synaptosomal fraction mediated by iron and ascorbate.
Rodney Levine,Joy Williams,Earl Stadtman,Emily Shacter (1994). [37] Carbonyl assays for determination of oxidatively modified proteins.
Carolyn Rennie,Stephen Thompson,Alistair Parker,Alun Maddy (1979). Human erythrocyte fractionation in “percoll” density gradients.
P Leaverton (1995). Statistical Inference: Tests for Statistical Significance, In: A Review of Biostatistics, A program for Self-Instruction.
A Amici,R Levine,L Tsai,E Stadtman (1989). Conversion of amino acid residues in proteins and amino acid homopolymers to carbonyl derivatives by metal-catalyzed oxidation reactions.
Hans Lutz (2012). Naturally Occurring Autoantibodies in Mediating Clearance of Senescent Red Blood Cells.
G Piccini,G Minetti,C Balduini,A Brovelli (1995). Oxidation state of glutathione and membrane proteins in human red cells of different age.
R Kannan,J Yuan,P Low (1991). Isolation and partial characterization of antibody- and globin-enriched complexes from membranes of dense human erythrocytes.
U Laemmli (1970). Cleavage of structural proteins during assembly of head of bacteriophage.
T Unknown Title.
A Habeeb (1972). [37] Reaction of protein sulfhydryl groups with Ellman's reagent.
J Singer,L Jennings,C Jackson,M Dockter,M Morrison,W Walker (1986). Erythrocyte homeostasis: antibody-mediated recognition of the senescent state by macrophages..

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No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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How to Cite This Article

Arghya Sur, Hemontika Chakraborty, Arindam Basu. 2014. "Oxidative Stress Induced Carbonyl Group Incorporation to Human RBC Membrane: Role in Vivo Senescence of Erythrocyte.". Global Journal of Medical Research, Global Journal of Medical Research - C: Microbiology & Pathology GJMR-C Volume 14 (GJMR Volume 14 Issue C7).

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GJMR Volume 14 Issue C7

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Journal Specifications

Crossref Journal DOI 10.17406/gjmr

Print ISSN 0975-5888

e-ISSN 2249-4618

Keywords

autoantibody to RBC human RBC membrane oxidative membrane damage oxygen free radical protein carbonylation ROS senescence of RBC

Classification

GJMR-C Classification NLMC Code: WH 150

Submission ReceivedDecember 15, 2013
Peer Review Double Blind
Handling Editor
Accepted December 31, 2013
Published January 15, 2014

Version of record

v1.2

Issue date

December 24, 2014

Language

English

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Total Score: 144

Country: India

Subject: Global Journal of Medical Research

Authors: Arghya Sur, Hemontika Chakraborty, Arindam Basu, Sasanka Chakrabarti (PhD/Dr. count: 0)

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