Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

Marcelo Vidigal Caliari

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Fabricio Marcus Silva Oliveira

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Bernardo Coelho Horta

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Luana Oliveira Prata

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Andrezza Fernanda Santiago

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Universidade Federal de Minas Gerais

Participation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice

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Abstract

Studies have shown that CD1 double negative mice (CD1d -/-) develop larger liver abscesses due to their inability to present amebic antigens to NK T lymphocytes. Therefore, we conducted flow cytometry studies to determine the frequency of NK T, CD4 + T, CD8 + T and B lymphocytes in mice with amebic colitis. The frequency of NK T, CD8 + T and B lymphocytes was reduced in the MLN of mice in the CTRL-CD1 -/-and Eh-CD1 -/-groups compared to the CTRL-WT and Eh-WT groups. There was also a significant decrease in the frequency of B lymphocytes in the spleens of the animals in the Eh-WT group when compared with the CTRL-CD1 -/-, Eh-CD1 -/- and CTRL-WT groups. The results of the flow cytometry analysis highlight the importance of NK T lymphocytes in the immune response of mice to amebic intestinal infection and the importance of CD1 molecules in the activation of T and B lymphocytes.

References

22 Cites in Article

Reference Format

(1997). WHO Expert Committee on Drug Dependence: Twenty-eighth report..
J Stanley,Sl (2003). Amoebiasis.
M Brigl,L Bry,S Kent (2003). Mechanism of CD1drestricted natural killer T cell activation during microbial infection.
S Moody,S Becker,Y Nuchamowitz,D Mirelman (1998). Identification of significant variation in the composition of lipophosphoglycan-like molecules of E. histolytica and E. dispar.
Fms Oliveira,B Coelho,L Prata (2012). Susceptibility to intestinal infection is related to reduction in natural killer T-lymphocytes in C57BL/6 mice.
S Smiley,P Lanthier,K Couper (2005). Exacerbated Susceptibility to Infection-Stimulated Immunopathology in CD1d-Deficient Mice.
H Macdonald (1995). NK1.1+ T cell receptor-alpha/beta+ cells: new clues to their origin, specificity, and function..
A Bendelac,M Rivera,S Park,J Roark (1997). Mouse CD1 specific NK1 T cells: development, specificity, an function.
M Kronenberg (2007). Toward an understanding of NKT cell biology: progress and paradoxes.
H Lotter,N González-Roldán,B Lindner (2009). Natural Killer T cells activated by a lipopeptidophosphoglycan from Entamoeba histolytica are critically important to control amebic liver abscess.
Emmanuel Tupin,Yuki Kinjo,Mitchell Kronenberg (2007). The unique role of natural killer T cells in the response to microorganisms.
Edward Nieuwenhuis,Tetsuya Matsumoto,Dicky Lindenbergh,Rob Willemsen,Arthur Kaser,Ytje Simons-Oosterhuis,Sylvia Brugman,Keizo Yamaguchi,Hiroki Ishikawa,Yuji Aiba,Yasuhiro Koga,Janneke Samsom,Kenshiro Oshima,Mami Kikuchi,Johanna Escher,Masahira Hattori,Andrew Onderdonk,Richard Blumberg (2009). Cd1d-dependent regulation of bacterial colonization in the intestine of mice.
H Arase,N Arase,Ogasawara (1992). An NK1.1 + CD4 + single-positive thymocyte subpopulation that expresses a highly skewed T cell antigen receptor family.
A Bendelac,R Schwartz CD4C and CD8C T cells acquire specific lymphokine secretion potentials during thymic maturation.
M Gomes,J Pesquero,C Furst (1999). An improved method to distinguish Entamoeba histolytica and Entamoeba dispar.
A Martinez,M Gomes,J Viana (1996). Isoenzyme profile as parameter to differentiate pathogenic strains of Entamoeba histolytica in Brazil.
N Ueda,H Kuki,D Kamimura (2006). CD1d-restricted NKT cell activation enhanced homeostatic proliferation of CD8+ T cells in a manner dependent on IL-4.
J Ventura‐juárez,L Barba‐gallardo,L Muñoz‐fernández,L Martínez‐medina,F Márquez‐díaz,S Sosa‐díaz,M Gerardo‐rodríguez,R González‐romo,R Campos‐rodríguez (2007). Immunohistochemical characterization of human fulminant amoebic colitis.
A Asgharpour,C Gilchirist,D Baba (2005). Resistance to Intestinal Entamoeba histolytica Infection is conferred by Innate Immunity and Gr-1 + Cells.
H Kitamura,A Otha,M Sekimoto,M Sato (2000). αgalactosylceramide induces early B-cell activation through IL-4 production by NKT cells.
G Galli,S Nuti,S Tavarini (2003). CD1d-restricted help to B cells by human invariant natural Killer T lymphocytes.
J Carrero,C Cervantes‐rebolledo,H Aguilar‐díaz,M Díaz‐gallardo,J Laclette,J Morales‐montor (2007). The role of the secretory immune response in the infection by <i>Entamoeba histolytica</i>.

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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How to Cite This Article

Marcelo Vidigal Caliari. 2015. \u201cParticipation of T, B and NKT Lymphocytes and CD1 Molecule in the Infection by Entamoeba histolytica in Mice\u201d. Global Journal of Medical Research - F: Diseases GJMR-F Volume 15 (GJMR Volume 15 Issue F4): .

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GJMR Volume 15 Issue F4
Pg. 7- 13

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-F Classification: NLMC Code: WC 524

Submission ReceivedApril 15, 2015
Peer Review Double Blind
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Accepted May 5, 2015
Published May 15, 2015

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November 2, 2015

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Article Matrices

Total Score: 105

Country: Brazil

Subject: Global Journal of Medical Research - F: Diseases

Authors: Marcelo Vidigal Caliari, Fabricio Marcus Silva Oliveira, Bernardo Coelho Horta, Luana Oliveira Prata, Andrezza Fernanda Santiago (PhD/Dr. count: 0)

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