Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

Sonia A Rizk

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Hany I Abu-Elnaga

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Akram Z Hegazy

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Veterinary Serum and Vaccine Research Institute

Performance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses

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Abstract

Proficient application of the advanced technology for foot-and-mouth disease virus (FMDV) detection, still a target challenge in Egypt, not only for rapid, identification of the transboundary continuously evolving virus, but also support accurate control strategy involving manufacturing of a highquality protective vaccine. The article discussed both two real-time RT-PCR (rRT-PCR) methods depending on either fluorophore or fluorescent dye for the precise detection of FMDV few copies. TaqMan based probe/primer overcame SYBR Green-based primer rRT-PCR by 10-fold for detecting less FMDV nucleic acid. In contrary, lower threshold cycles values with fewer nucleic acid copies biased to the fluorescent RRT-PCR with 3-4 cycles earlier. TaqMan format had better sensitivity and specificity, while SYBR Green method displayed less detection limit and precision.

References

11 Cites in Article

Reference Format

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

Ethical Approval

No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

Sonia A Rizk. 2018. \u201cPerformance of various TaqMan and SYBR Green rRT-PCR methods in detecting the Egyptian foot-and-mouth disease viruses\u201d. Global Journal of Medical Research - G: Veterinary Science & Medicine GJMR-G Volume 18 (GJMR Volume 18 Issue G2).

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GJMR Volume 18 Issue G2

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Journal Specifications

Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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GJMR-G Classification NLMC Code: WC 900

Submission ReceivedDecember 8, 2017
Peer Review Double Blind
Handling Editor
Accepted December 31, 2017
Published January 15, 2018

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v1.2

Issue date

September 29, 2018

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Article Matrices

Total Score: 103

Country: Egypt

Subject: Global Journal of Medical Research - G: Veterinary Science & Medicine

Authors: Hany I Abu-Elnaga, Sonia A Rizk, Akram Z Hegazy (PhD/Dr. count: 0)

View Count (all-time): 163

Total Views (Real + Logic): 3203

Total Downloads (simulated): 1472

Publish Date: 2018 09, Sat

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Total Views: 3203

Total Downloads: 1472

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