Physiochemical and Functional Characterization of a Dominant Grain Endosperm Protein Called Glutelin in Rice (Oryza Sativa L.) Using in Silico Methods

Physiochemical and Functional Characterization of a Dominant Grain Endosperm Protein Called Glutelin in Rice (Oryza Sativa L.) Using in Silico Methods

MNV Prasad Gajula

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E. Ramprasad

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Ch. V. Durga Rani

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G. Padmavathi

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S. Vanisri

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α Professor Jayashankar Telangana State Agricultural University

Physiochemical and Functional Characterization of a Dominant Grain Endosperm Protein Called Glutelin in Rice (Oryza Sativa L.) Using in Silico Methods

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Abstract

Glutelin protein is the most well-known abundant seed storage protein in rice seed endosperm. A total of 9 glutelin and glutelin type protein sequences from Oryza species available in uniport were evaluated by using bioinformatics tools to investigate physico-chemical properties, secondary structure prediction, putative phosphorylation sites and conserved motif search. Physicochemical analysis offers data such as pI, EC, Al, GRAVY and II about these sequences and the results showed that all glutelin protein sequences are basic, hydrophilic, thermo stable, having some extracellular portion. The secondary structure of the protein sequences were also predicted using SOPMA server. It was observed that alpha helix was predominant, followed by random coil, extended strand and least beta turn was found. Putative phosphorylation sites were also identified which are found to be conserved in plant species and the results showed that the most abundant phosphorylation site is serine residues in glutelin protein sequences.

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11 Cites in Article

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Funding

No external funding was declared for this work.

Conflict of Interest

The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

Data Availability

Not applicable for this article.

How to Cite This Article

MNV Prasad Gajula. 2017. \u201cPhysiochemical and Functional Characterization of a Dominant Grain Endosperm Protein Called Glutelin in Rice (Oryza Sativa L.) Using in Silico Methods\u201d. Global Journal of Science Frontier Research - I: Interdisciplinary GJSFR-I Volume 16 (GJSFR Volume 16 Issue I3): .

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GJSFR Volume 16 Issue I3
Pg. 43- 48

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Journal Specifications

Crossref Journal DOI 10.17406/GJSFR

Print ISSN 0975-5896

e-ISSN 2249-4626

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GJSFR-I Classification: FOR Code: 060799

Submission ReceivedNovember 3, 2016
Peer Review Double Blind
Handling Editor
Accepted November 30, 2016
Published December 13, 2016

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v1.2

Issue date

January 25, 2017

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Article Matrices

Total Score: 105

Country: India

Subject: Global Journal of Science Frontier Research - I: Interdisciplinary

Authors: E. Ramprasad, MNV Prasad Gajula, Ch. V. Durga Rani, G. Padmavathi, S. Vanisri (PhD/Dr. count: 0)

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Publish Date: 2017 01, Wed

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Total Views: 3603

Total Downloads: 1832

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