Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

1
Kashinath R.T.
Kashinath R.T. Msc Biochemistry, Ph.D. Biochemistry (Medical Faculty), FICS
2
Nagendra .S.
Nagendra .S.
3
Rudrappa. G.
Rudrappa. G.
4
Srinivas.S.
Srinivas.S.
1 Rajive Gandhi University of Health Science, Bangalore.

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Generally, in fasting state, gluconeogenesis accounts for about 30% of overall hepatic glucose output, the increase in type 2 diabetic subjects may be much more than this level. There is a close relationship between glucose metabolism and amino acid metabolism which is established through transamination reactions. The key amino acid -keto acid pairs involved in transamination reactions are glutamateα ketoglutarate, aspartate -oxaloacetate and pyruvate -alanine. A study was undertaken to assess the plasma levels of total amino acids, glutamate and alanine in type 2 diabetic subjects. A blood sample (5ml) with heparin as an anticoagulant was collected in the fasting state, from each of the selected normal as well as diabetic subjects. The separated plasma samples were employed for the estimation of total amino acid nitrogen levels, as well as for the estimation of plasma alanineand glutamate levels. The results indicate a significant rise in the plasma levels of total amino acids, alanine & glutamate in type-2 diabetic subjects as well as a significant increase in plasma alanine levels in diabetics of 30-40 yrs age as compared to diabetics of 41-60yrs of age. These findings suggest an increased availability of glucogenic amino acid precursors for glucose formation may be due to lack of proteolytic suppression of insulin.

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No external funding was declared for this work.

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The authors declare no conflict of interest.

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No ethics committee approval was required for this article type.

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Not applicable for this article.

Kashinath R.T.. 2014. \u201cPlasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects\u201d. Global Journal of Medical Research - K: Interdisciplinary GJMR-K Volume 14 (GJMR Volume 14 Issue K1): .

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Crossref Journal DOI 10.17406/gjmra

Print ISSN 0975-5888

e-ISSN 2249-4618

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March 13, 2014

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English

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Generally, in fasting state, gluconeogenesis accounts for about 30% of overall hepatic glucose output, the increase in type 2 diabetic subjects may be much more than this level. There is a close relationship between glucose metabolism and amino acid metabolism which is established through transamination reactions. The key amino acid -keto acid pairs involved in transamination reactions are glutamateα ketoglutarate, aspartate -oxaloacetate and pyruvate -alanine. A study was undertaken to assess the plasma levels of total amino acids, glutamate and alanine in type 2 diabetic subjects. A blood sample (5ml) with heparin as an anticoagulant was collected in the fasting state, from each of the selected normal as well as diabetic subjects. The separated plasma samples were employed for the estimation of total amino acid nitrogen levels, as well as for the estimation of plasma alanineand glutamate levels. The results indicate a significant rise in the plasma levels of total amino acids, alanine & glutamate in type-2 diabetic subjects as well as a significant increase in plasma alanine levels in diabetics of 30-40 yrs age as compared to diabetics of 41-60yrs of age. These findings suggest an increased availability of glucogenic amino acid precursors for glucose formation may be due to lack of proteolytic suppression of insulin.

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Plasma Total Amino Acids, Plasma Glutamate and Alanine Levels in Diabetic Subjects

Kashinath R.T.
Kashinath R.T. Rajive Gandhi University of Health Science, Bangalore.
Nagendra .S.
Nagendra .S.
Rudrappa. G.
Rudrappa. G.
Srinivas.S.
Srinivas.S.

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